DNAJB11 Polyclonal antibody

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  WB analysis using 15484-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15484-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15484-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15484-1-AP (DNAJB11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15484-1-AP

  IP result of anti-DNAJB11 (IP:15484-1-AP, 4ug; Detection:15484-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human ovary tumor using 15484-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 15484-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15484-1-AP (DNAJB11 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15484-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJB11 antibody 15484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

DNAJB11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15484-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ABBP 2, ABBP2, APOBEC1 binding protein 2, DJ9, DnaJ protein homolog 9, DNAJB11, EDJ, ER associated DNAJ, ER associated dnaJ protein 3, ERdj3, ERj3, ERj3p, hDj 9, hDj9, HEDJ, Human DnaJ protein 9, PRO1080, PWP1 interacting protein 4, UNQ537, UNQ537/PRO1080

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse heart tissue, HepG2 cells, rat liver tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

15484-1-AP targets DNAJB11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7832

  Product name: Recombinant human DNAJB11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-358 aa of BC001144

  Sequence: GRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQGY

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 11
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC001144
• Gene Symbol: DNAJB11
• Gene ID (NCBI): 51726
• RRID: AB_2094400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBS4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Science; The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.; Authors - Michael J McKenna
human	WB	Cell; Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection.; Authors - Shuhei Taguwa ; KD Validated
mouse	WB	Mol Cell; Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network.; Authors - Willianne I M Vonk
human	WB, IP	EMBO J; Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis.; Authors - Joseph C Genereux ; KD Validated
human	WB	Cell Chem Biol; Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.; Authors - Lars Plate
human	WB	Cell Death Dis; Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II.; Authors - Yosuke Osaki ; KD Validated