DNAJB2 Monoclonal antibody

DNAJB2 Monoclonal Antibody for WB, ELISA

Cat No. 60204-1-Ig

Clone No. 2G8C5

Publications (1)

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Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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WB analysis of SH-SY5Y using 60204-1-Ig
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60204-1-Ig (DNAJB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for DNAJB2 in ?

Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Tested Applications

Positive WB detected in

SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60204-1-Ig targets DNAJB2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag1271 Product name: Recombinant human DNAJB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-324 aa of BC011609 Sequence: MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL Predict reactive species
Full Name	DnaJ (Hsp40) homolog, subfamily B, member 2
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	80 kDa
GenBank Accession Number	BC011609
Gene Symbol	DNAJB2
Gene ID (NCBI)	3300
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P25686
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The DNAJB2 Monoclonal antibody (2G8C5), catalog number 60204-1-Ig, has 1 citation to date. It appears in the journal Inflammation (2025), in a study investigating how DNAJB2 attenuates rosacea skin inflammation and angiogenesis via the endoplasmic reticulum stress-mediated TLR2/Myd88/NF-κB pathway. The research field encompasses immunology and inflammatory skin disease, with the antibody applied in Western Blot using mouse samples.

Species	Application	Title
mouse	WB	Inflammation DNAJB2 Attenuates Rosacea Skin Inflammation and Angiogenesis by Inhibiting the Endoplasmic Reticulum Stress-mediated TLR2/Myd88/NF-κB pathway Authors - Yuxin Qing View Article