DNAJB4 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJB4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13064-1-AP

Synonyms

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein

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Validation Data Gallery

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  WB analysis using 13064-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13064-1-AP

  Mouse serum lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13064-1-AP

  IP result of anti-DNAJB4 (IP:13064-1-AP, 4ug; Detection:13064-1-AP 1:2000) with HeLa cells lysate 2600 ug.

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  IHC staining of human pancreas cancer using 13064-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13064-1-AP (DNAJB4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 13064-1-AP

  Immunofluorescent analysis of MCF-7 cells, using DNAJB4 antibody 13064-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13064-1-AP targets DNAJB4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJB4 fusion protein Ag3756
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 4
• Calculated molecular weight: 38 kDa
• Observed molecular weight: 38 kDa
• GenBank accession number: BC034721
• Gene symbol: DNAJB4
• Gene ID (NCBI): 11080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF	Acta Neuropathol; Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4; Authors - Michio Inoue
human	WB	OMICS; HLJ1 (DNAJB4) Gene Is a Novel Biomarker Candidate in Breast Cancer.; Authors - Tolga Acun
human	WB,IHC	Int J Clin Exp Pathol; HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival.; Authors - Yong Liu
mouse	WB	Int J Mol Sci; Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.; Authors - Man-Chen Hsu ; KO Validated
mouse	WB	Elife; HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages; Authors - Wei-Jia Luo ; KD Validated
mouse	WB	Acta Neuropathol; Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure; Authors - Conrad C Weihl ; KO Validated