DNAJB4 Polyclonal antibody

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• DNAJB4 Polyclonal antibody (13064-1-AP)

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• KD/KO Validated

DNAJB4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13064-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

DjB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog

• 

  WB analysis using 13064-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13064-1-AP

  Mouse serum lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 13064-1-AP

  IP result of anti-DNAJB4 (IP:13064-1-AP, 4ug; Detection:13064-1-AP 1:2000) with HeLa cells lysate 2600 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 13064-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13064-1-AP (DNAJB4 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 13064-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DNAJB4 antibody (13064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13064-1-AP targets DNAJB4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3756

  Product name: Recombinant human DNAJB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-337 aa of BC034721

  Sequence: MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 4
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC034721
• Gene Symbol: DNAJB4
• Gene ID (NCBI): 11080
• RRID: AB_2094422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UDY4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-DNAJB4 (HLJ1) antibody (Cat# 13064-1-AP) has 13 citations across journals including Acta Neuropathologica, EMBO Journal, eLife, Molecular Therapy Nucleic Acids, Journal of Virology, Cell Biology and Toxicology, International Journal of Molecular Sciences, Cell Oncology, Clinical and Experimental Medicine, Discover Oncology, OMICS, and International Journal of Clinical and Experimental Pathology. Research fields span neuromuscular disease, circadian biology, hepatocarcinogenesis, breast cancer, colorectal carcinoma, renal cell carcinoma, hepatitis B, and sepsis/inflammation.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IF	Acta Neuropathol; Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4; Authors - Michio Inoue
mouse	WB	Acta Neuropathol; Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure; Authors - Conrad C Weihl ; KO Validated
mouse	WB,IP,IF	EMBO J; O-GlcNAcylation of circadian clock protein Bmal1 impairs cognitive function in diabetic mice; Authors - Ya Hui
mouse	WB	Mol Ther Nucleic Acids; DNAJB6 isoform specific knockdown: Therapeutic potential for limb girdle muscular dystrophy D1; Authors - Andrew R Findlay
mouse,human	WB	Cell Biol Toxicol; DNAJB4/HLJ1 deficiency sensitizes diethylnitrosamine-induced hepatocarcinogenesis with peritumoral STAT3 activation; Authors - Wei-Jia Luo
mouse	WB	Int J Mol Sci; Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.; Authors - Man-Chen Hsu ; KO Validated