DNAJB4 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJB4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 13064-1-AP

Synonyms

Human liver DnaJ like protein, HSP40 homolog, HLJ1, Heat shock protein 40 homolog, DNAJW

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Validation Data Gallery

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  WB analysis using 13064-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13064-1-AP

  Mouse serum lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13064-1-AP

  IP result of anti-DNAJB4 (IP:13064-1-AP, 4ug; Detection:13064-1-AP 1:2000) with HeLa cells lysate 2600 ug.

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  IHC staining of human pancreas cancer using 13064-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13064-1-AP (DNAJB4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 13064-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DNAJB4 antibody (13064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

13064-1-AP targets DNAJB4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJB4 fusion protein Ag3756
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 4
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC034721
• Gene Symbol: DNAJB4
• Gene ID (NCBI): 11080
• RRID: AB_2094422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IF	Acta Neuropathol; Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4; Authors - Michio Inoue
mouse	WB	Int J Mol Sci; Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.; Authors - Man-Chen Hsu ; KO Validated
human	WB	OMICS; HLJ1 (DNAJB4) Gene Is a Novel Biomarker Candidate in Breast Cancer.; Authors - Tolga Acun
human	WB,IHC	Int J Clin Exp Pathol; HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival.; Authors - Yong Liu
mouse	WB	Elife; HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages; Authors - Wei-Jia Luo ; KD Validated
mouse	WB	Acta Neuropathol; Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure; Authors - Conrad C Weihl ; KO Validated