DNAJB4 Polyclonal antibody

DNAJB4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13064-1-AP

Synonyms

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein



Tested Applications

Positive WB detected inHeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13064-1-AP targets DNAJB4 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB4 fusion protein Ag3756
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC034721
Gene symbol DNAJB4
Gene ID (NCBI) 11080
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB4 antibody 13064-1-APDownload protocol
IHC protocol for DNAJB4 antibody 13064-1-APDownload protocol
IF protocol for DNAJB4 antibody 13064-1-APDownload protocol
IP protocol for DNAJB4 antibody 13064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Clin Exp Pathol

HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival.

Authors - Yong Liu
humanWB

OMICS

HLJ1 (DNAJB4) Gene Is a Novel Biomarker Candidate in Breast Cancer.

Authors - Tolga Acun