DNAJB5 Polyclonal antibody

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  WB analysis of rat heart using 16453-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16453-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16453-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16453-1-AP (DNAJB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16453-1-AP

  IP result of anti-DNAJB5 (IP:16453-1-AP, 4ug; Detection:16453-1-AP 1:500) with HepG2 cells lysate 3300 ug.

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  IHC staining of human heart using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16453-1-AP

  Immunofluorescent analysis of HepG2 cells using 16453-1-AP (DNAJB5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DNAJB5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16453-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIAA1045, Hsc40, Heat shock protein Hsp40-3, Heat shock protein Hsp40-2, Heat shock protein Hsp40 3

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Tested Applications

• Positive WB detected in: rat heart tissue, HeLa cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human heart tissue, human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16453-1-AP targets DNAJB5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9341

  Product name: Recombinant human DNAJB5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-386 aa of BC012115

  Sequence: MGKDYYKILGIPSGANEDEIKKAYRKMALKYHPDKNKEPNAEEKFKEIAEAYDVLSDPKKRGLYDQYGEEGLKTGGGTSGGSSGSFHYTFHGDPHATFASFFGGSNPFDIFFASSRSTRPFSGFDPDDMDVDEDEDPFGAFGRFGFNGLSRGPRRAPEPLYPRRKVQDPPVVHELRVSLEEIYHGSTKRMKITRRRLNPDGRTVRTEDKILHIVIKRGWKEGTKITFPKEGDATPDNIPADIVFVLKDKPHAHFRRDGTNVLYSALISLKEALCGCTVNIPTIDGRVIPLPCNDVIKPGTVKRLRGEGLPFPKVPTQRGDLIVEFKVRFPDRLTPQTRQILKQQYPQHSLHSMCTQLDVHWTLATFSKMQKKATGFQENVPVPDPF

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 5
• Calculated Molecular Weight: 386 aa, 44 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC012115
• Gene Symbol: DNAJB5
• Gene ID (NCBI): 25822
• RRID: AB_2094724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75953
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJB5, also named as Hsc40, is an Hsp40 Family Protein with MW 39-40 kDa. It mediates antihypertrophic effects of Thioredoxin1 in the heart. This protein has some isoforms with MW 39 kDa and 47-51 kDa.