DNAJB8 Recombinant monoclonal antibody

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• DNAJB8 Recombinant monoclonal antibody (86763-3-RR)

• 

  WB analysis using 86763-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86763-3-RR (DNAJB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86763-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86763-3-RR (DNAJB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DNAJB8 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86763-3-RR

Clone No.251752B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DJ6

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Tested Applications

• Positive WB detected in: SW480 cells, mouse liver tissue, HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86763-3-RR targets DNAJB8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10899

  Product name: Recombinant human DNAJB8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-232 aa of BC029521

  Sequence: MANYYEVLGVQASASPEDIKKAYRKLALRWHPDKNPDNKEEAEKKFKLVSEAYEVLSDSKKRSLYDRAGCDSWRAGGGASTPYHSPFDTGYTFRNPEDIFREFFGGLDPFSFEFWDSPFNSDRGGRGHGLRGAFSAGFGEFPAFMEAFSSFNMLGCSGGSHTTFSSTSFGGSSSGSSGFKSVMSSTEMINGHKVTTKRIVENGQERVEVEEDGQLKSVTVNGKEQLKWMDSK

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 8
• Calculated Molecular Weight: 232 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC029521
• Gene Symbol: DNAJB8
• Gene ID (NCBI): 165721
• RRID: AB_3745108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NHS0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJB8, a member of the DNAJ/HSP40 family of proteins, plays a crucial role in regulating chaperone HSP70 activity. It is known for its ability to suppress the aggregation of polyglutamine proteins through its C-terminal tail. Furthermore, DNAJB8 has been shown to inhibit TP53 through ubiquitin degradation in human colon cancer by directly interacting and upregulating MDR1, leading to increased oxaliplatin resistance. High levels of DNAJB8 in both colon tissues and serum are correlated with worse overall survival of colon cancer patients, suggesting that DNAJB8 level in serum sEV may serve as a potential biomarker for colon cancer and a promising therapeutic target for oxaliplatin-resistant cancer.