Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, SH-SY5Y cells, HepG2 cells, mouse liver tissue|
|Positive IHC detected in||human liver tissue, human skeletal muscle tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17331-1-AP targets DNAJC11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNAJC11 fusion protein Ag11310|
|Full Name||DnaJ (Hsp40) homolog, subfamily C, member 11|
|Calculated molecular weight||507 aa, 57 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC014145|
|Gene ID (NCBI)||55735|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DNAJC11 is a member of the J proteins also known as HSP40 family of co-chaperones. Recently DNAJC11 has been found to interact with mitofilin, a mitochondrial inner membrane protein, indicating that it may be a molecular chaperone during mitochondrial protein import and folding.
J Proteome Res
Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.
A Splicing Mutation in the Novel Mitochondrial Protein DNAJC11 Causes Motor Neuron Pathology Associated with Cristae Disorganization, and Lymphoid Abnormalities in Mice.
Nat Cell Biol
Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space