DNAJC11 Polyclonal antibody

DNAJC11 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17331-1-AP

Synonyms

DNAJC11



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, SH-SY5Y cells
Positive IHC detected inhuman skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 17331-1-AP is DNAJC11 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC11 fusion protein Ag11310
Full Name DnaJ (Hsp40) homolog, subfamily C, member 11
Calculated molecular weight 507 aa, 57 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC014145
Gene symbol DNAJC11
Gene ID (NCBI) 55735
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJC11 is a member of the J proteins also known as HSP40 family of co-chaperones. Recently DNAJC11 has been found to interact with mitofilin, a mitochondrial inner membrane protein, indicating that it may be a molecular chaperone during mitochondrial protein import and folding.

Protocols

Product Specific Protocols
WB protocol for DNAJC11 antibody 17331-1-APDownload protocol
IHC protocol for DNAJC11 antibody 17331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

A Splicing Mutation in the Novel Mitochondrial Protein DNAJC11 Causes Motor Neuron Pathology Associated with Cristae Disorganization, and Lymphoid Abnormalities in Mice.

Authors - Fotis Ioakeimidis
humanWB

J Proteome Res

Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.

Authors - Foteini Violitzi