Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse liver tissue, SH-SY5Y cells|
|Positive IHC detected in||human skeletal muscle tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17331-1-AP is DNAJC11 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNAJC11 fusion protein Ag11310|
|Full Name||DnaJ (Hsp40) homolog, subfamily C, member 11|
|Calculated molecular weight||507 aa, 57 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC014145|
|Gene ID (NCBI)||55735|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DNAJC11 is a member of the J proteins also known as HSP40 family of co-chaperones. Recently DNAJC11 has been found to interact with mitofilin, a mitochondrial inner membrane protein, indicating that it may be a molecular chaperone during mitochondrial protein import and folding.
A Splicing Mutation in the Novel Mitochondrial Protein DNAJC11 Causes Motor Neuron Pathology Associated with Cristae Disorganization, and Lymphoid Abnormalities in Mice.
J Proteome Res
Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.