|Positive WB detected in||A549 cells|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12338-1-AP targets DNAJC12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNAJC12 fusion protein Ag2989|
|Full Name||DnaJ (Hsp40) homolog, subfamily C, member 12|
|Calculated molecular weight||198 aa, 23 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC017018|
|Gene ID (NCBI)||56521|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ann Surg Oncol
Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.
Cell Stress Chaperones
The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress.