DNAJC12 Polyclonal antibody

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• KD/KO Validated

DNAJC12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12338-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

DNAJC12, J domain containing protein 1, JDP1

• 

  WB analysis using 12338-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12338-1-AP (DNAJC12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of BxPC-3 using 12338-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12338-1-AP (DNAJC12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 12338-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12338-1-AP (DNAJC12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 12338-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12338-1-AP (DNAJC12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12338-1-AP targets DNAJC12 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2989

  Product name: Recombinant human DNAJC12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-198 aa of BC017018

  Sequence: MDAILNYRSEDTEDYYTLLGCDELSSVEQILAEFKVRALECHPDKHPENPKAVETFQKLQKAKEILTNEESRARYDHWRRSQMSMPFQQWEALNDSVKTSMHWVVRGKKDLMLEESDKTHTTKMENEECNEQRERKKEELASTAEKTEQKEPKPLEKSVSPQNSDSSGFADVNGWHLRFRWSKDAPSELLRKFRNYEI

• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 12
• Calculated Molecular Weight: 198 aa, 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC017018
• Gene Symbol: DNAJC12
• Gene ID (NCBI): 56521
• RRID: AB_2246188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKB3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Anti-DNAJC12 antibody (Cat# 12338-1-AP) has 7 total citations. Citations appear in Cell Reports Medicine, NPJ Parkinson's Disease, Journal of Molecular Cell Biology, Cell Stress & Chaperones, Annals of Surgical Oncology, Frontiers in Oncology, and bioRxiv. Research fields span biliary tract cancer organoid drug screening, Parkinson's disease and dopaminergic dysfunction, neuroblastoma progression via histone lactylation, ER stress and co-chaperone biology, gastric cancer aggressiveness, breast carcinoma signaling, and Dnajc12 knockout mouse characterization.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
human	WB	NPJ Parkinsons Dis; Central biogenic amine deficiency with concomitant exploratory behavioral deficits in Dnajc12 knock-out mice; Authors - Isaac Bul Deng ; KD Validated
human	WB	J Mol Cell Biol; DNAJC12 downregulation induces neuroblastoma progression via increased histone H4K5 lactylation; Authors - Yaqi Yang ; KO Validated
human	WB,IF	Cell Stress Chaperones; The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress.; Authors - Choi Jin J
human	WB	Ann Surg Oncol; Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.; Authors - Yasuo Uno ; KD Validated
human	WB	Front Oncol; DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.; Authors - Mianjie Lin