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  • KD/KO Validated

DNAJC12 Polyclonal antibody

DNAJC12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12338-1-AP

Synonyms

DNAJC12, J domain containing protein 1, JDP1



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12338-1-AP targets DNAJC12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC12 fusion protein Ag2989
Full Name DnaJ (Hsp40) homolog, subfamily C, member 12
Calculated molecular weight 198 aa, 23 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC017018
Gene symbol DNAJC12
Gene ID (NCBI) 56521
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJC12 antibody 12338-1-APDownload protocol
IHC protocol for DNAJC12 antibody 12338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Ann Surg Oncol

Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.

Authors - Yasuo Uno
  • KD Validated
humanWB,IF

Cell Stress Chaperones

The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress.

Authors - Choi Jin J