DNAJC12 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJC12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12338-1-AP

Synonyms

DNAJC12, J domain containing protein 1, JDP1

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Validation Data Gallery

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  WB analysis of BxPC-3 using 12338-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12338-1-AP (DNAJC12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12338-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12338-1-AP (DNAJC12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 12338-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12338-1-AP (DNAJC12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12338-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12338-1-AP (DNAJC12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12338-1-AP targets DNAJC12 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC12 fusion protein Ag2989
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 12
• Calculated Molecular Weight: 198 aa, 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC017018
• Gene Symbol: DNAJC12
• Gene ID (NCBI): 56521
• RRID: AB_2246188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
human	WB	Front Oncol; DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.; Authors - Mianjie Lin
human	WB,IF	Cell Stress Chaperones; The co-chaperone DNAJC12 binds to Hsc70 and is upregulated by endoplasmic reticulum stress.; Authors - Choi Jin J
mouse	IF	bioRxiv; Characterization of Dnajc12 knockout mice, a model of hypodopaminergia; Authors - Isaac Bul Deng
human	WB	Ann Surg Oncol; Increased Expression of DNAJC12 is Associated with Aggressive Phenotype of Gastric Cancer.; Authors - Yasuo Uno ; KD Validated