DNAJC17 Polyclonal antibody

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  WB analysis using 31580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31580-1-AP (DNAJC17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DNAJC17 Polyclonal Antibody for WB, ELISA

Cat No. 31580-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FLJ10634

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31580-1-AP targets DNAJC17 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35992

  Product name: Recombinant human DNAJC17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 69-194 aa of BC000048

  Sequence: RAAYDKVRKAKKQAAERTQKLDEKRKKVKLDLEARERQAQAQESEEEEESRSTRTLEQEIERLREEGSRQLEEQQRLIREQIRQERDQRLRGKAENTEGQGTPKLKLKWKCKKEDESKGGYSKDVL

• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 17
• Calculated Molecular Weight: 304 aa, 35 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000048
• Gene Symbol: DNAJC17
• Gene ID (NCBI): 55192
• RRID: AB_3670044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NVM6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DnaJ proteins, also known as Hsp40, are a set of highly conserved proteins typically acting as co-factors of Hsp70 molecular chaperones. Based on their domain architecture, DNAJ is classified into three different groups (A, B, and C). DNAJC17 belongs to group C, bearing the J domain at its N-terminal region and an RNA recognition motif (RRM) located at the protein C-terminu. DNAJC17 is a susceptibility gene for congenital hypothyroidism and demonstrated its wide expression in mice since early embryogenesis, in which it plays an essential role in pre-implantation stages (PMID: 29773831).