DNAJC27 Polyclonal antibody

• 

  WB analysis using 17043-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17043-1-AP (DNAJC27 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17043-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17043-1-AP (DNAJC27 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

DNAJC27 Polyclonal Antibody for WB, ELISA

Cat No. 17043-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Rab and DnaJ domain-containing protein, RABJS, RBJ

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

17043-1-AP targets DNAJC27 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10586

  Product name: Recombinant human DNAJC27 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-273 aa of BC034049

  Sequence: MEANMPKRKEPGRSLRIKVISMGNAEVGKSCIIKRYCEKRFVSKYLATIGIDYGVTKVHVRDREIKVNIFDMAGHPFFYEVRNEFYKDTQGVILVYDVGQKDSFDALDAWLAEMKQELGPHGNMENIIFVVCANKIDCTKHRCVDESEGRLWAESKGFLYFETSAQTGEGINEMFQTFYISIVDLCENGGKRPTTNSSASFTKEQADAIRRIRNSKDSWDMLGVKPGASRDEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTALLKNIK

• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 27
• Calculated Molecular Weight: 273 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC034049
• Gene Symbol: DNAJC27
• Gene ID (NCBI): 51277
• RRID: AB_2095199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZQ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC27(DnaJ heat shock protein family member C27), also known as RabJS or RBJ, is a protein-coding gene located on human chromosome 2p23.3. It belongs to the DNAJ (HSP40) family, which is characterized by the presence of a highly conserved J-domain that interacts with HSP70 chaperones to regulate protein folding, stability, and degradation under both normal and stress conditions.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Curr Pharm Des; Non-canonical Small GTPase RBJ Promotes NSCLC Progression Through the Canonical MEK/ERK Signaling Pathway; Authors - Yujin Wang ; KD Validated