Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
17043-1-AP targets DNAJC27 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10586 Product name: Recombinant human DNAJC27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-273 aa of BC034049 Sequence: MEANMPKRKEPGRSLRIKVISMGNAEVGKSCIIKRYCEKRFVSKYLATIGIDYGVTKVHVRDREIKVNIFDMAGHPFFYEVRNEFYKDTQGVILVYDVGQKDSFDALDAWLAEMKQELGPHGNMENIIFVVCANKIDCTKHRCVDESEGRLWAESKGFLYFETSAQTGEGINEMFQTFYISIVDLCENGGKRPTTNSSASFTKEQADAIRRIRNSKDSWDMLGVKPGASRDEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTALLKNIK Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 27 |
| Calculated Molecular Weight | 273 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC034049 |
| Gene Symbol | DNAJC27 |
| Gene ID (NCBI) | 51277 |
| RRID | AB_2095199 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJC27(DnaJ heat shock protein family member C27), also known as RabJS or RBJ, is a protein-coding gene located on human chromosome 2p23.3. It belongs to the DNAJ (HSP40) family, which is characterized by the presence of a highly conserved J-domain that interacts with HSP70 chaperones to regulate protein folding, stability, and degradation under both normal and stress conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJC27 antibody 17043-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |



