DNAJC3 Polyclonal antibody

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  WB analysis using 26721-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26721-1-AP (DNAJC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26721-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26721-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 26721-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 26721-1-AP (DNAJC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

DNAJC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26721-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DNAJC3, HP58, P58, P58IPK, PRKRI, Protein kinase inhibitor p58

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human colon cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26721-1-AP targets DNAJC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24966

  Product name: Recombinant human DNAJC3 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 32-380 aa of BC047936

  Sequence: GVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYDEAIQDYETAQEHNENDQQIREGL

• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 3
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC047936
• Gene Symbol: DNAJC3
• Gene ID (NCBI): 5611
• RRID: AB_2880613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13217
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	FASEB J; UFMylation of HRD1 regulates endoplasmic reticulum homeostasis; Authors - Hui Luo
human	WB	FEBS Open Bio; A systems biological analysis of the ATF4-GADD34-CHOP regulatory triangle upon endoplasmic reticulum stress; Authors - Margita Márton