DNAJC5 Polyclonal antibody

• 

  WB analysis using 32204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32204-1-AP (DNAJC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 32204-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC5 antibody (32204-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

DNAJC5 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 32204-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DnaJ Heat Shock Protein Family (Hsp40) Member C5, DnaJ (Hsp40) Homolog, Subfamily C, Member 5, Cysteine string protein, CSP, CLN4

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32204-1-AP targets DNAJC5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC5 fusion protein Ag35443
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 5
• Calculated Molecular Weight: 22kDa，198aa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_025219.2
• Gene Symbol: DNAJC5
• Gene ID (NCBI): 80331
• RRID: AB_3670222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H3Z4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC5 (DnaJ homolog subfamily C member 5), also known as cysteine string protein α (CSPα), is a member of the HSP40 (DNAJ) family of proteins, as well as a co-chaperone of HSC70, and has been shown to control the extracellular release of many neurodegenerative disease proteins. DNAJC5 contains three structural domains - -a common N-terminal J structural domain conserved in DNAJ proteins, the cysteine string (CS) central structural domain, which is highly palmitoylated and anchors the protein to late endosomes, and an overall disordered C-terminal structural domain. Mutations in DNAJC5 have been strongly implicated in the development of human diseases such as neuronal waxy lipofuscinosis, cystic fibrosis, Huntington's disease and Parkinson's disease.