Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, Raji cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25444-1-AP targets DNAJC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22123 Product name: Recombinant human DNAJC9 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-260 aa of BC136507 Sequence: MGLLDLCEEVFGTADLYRVLGVRREASDGEVRRGYHKVSLQVHPDRVGEGDKEDATRRFQILGKVYSVLSDREQRAVYDEQGTVDEDSPVLTQDRDWEAYWRLLFKKISLEDIQAFEKTYKGSEEELADIKQAYLDFKGDMDQIMESVLCVQYTEEPRIRNIIQQAIDAGEVPSYNAFVKESKQKMNARKRRAQEEAKEAEMSRKELGLDEGVDSLKAAIQSRQKDRQKEMDNFLAQMEAKYCKSSKGGGKKSALKKEKK Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 9 |
| Calculated Molecular Weight | 260 aa, 30 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC136507 |
| Gene Symbol | DNAJC9 |
| Gene ID (NCBI) | 23234 |
| RRID | AB_2880084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJC9, DnaJ homolog subfamily C member 9, acts as a dual histone chaperone and heat shock co-chaperone (PMID: 33857403). As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers (PMID: 33857403). DNAJC9 also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins. (PMID: 17182002, PMID: 33857403). DNAJC9 exhibits activity to assemble histones onto DNA (PMID: 33857403). DNAJC9 is an essential protein in many cancer cell types and the levels of the protein correlate with the rates at which cancer cells proliferate.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJC9 antibody 25444-1-AP | Download protocol |
| IHC protocol for DNAJC9 antibody 25444-1-AP | Download protocol |
| WB protocol for DNAJC9 antibody 25444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNAJC9 Polyclonal antibody (catalog number 25444-1-AP) has 4 total citations. These appear in Nature Communications, Scientific Reports, Journal of Medical Virology, and bioRxiv. The associated research fields include chromatin assembly (ASF1/CDAN1 engagement and inhibition), proteomics (miR-26a-knockout HeLa cell protein expression analysis), and virology (DNAJC9's role in enhancing hepatitis B virus cccDNA transcription). All citations used the antibody in Western blot applications with human samples.
| Species | Application | Title |
|---|---|---|
Sci Rep Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology. | ||
J Med Virol DNAJC9 Binds to and Enhances the Transcription of Hepatitis B Virus cccDNA by Recruiting Histone H3.3 | ||
Reviews
What customers say
Both reviewers rated the antibody 5 stars for Western Blot. One validated specificity using CRISPR/CAS9 DNAJC9 knockout in human primary CD4+ T cells at a 1:1000 dilution. The other confirmed strong performance on human and mouse pancreatic cancer cell lines at 1:5000 dilution, with specificity verified by DNAJC9 knockout cells. Overall, users report reliable specificity and clear signal across species and cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
HELENA (Verified Customer) (04-26-2023) | Samples correspond to activated human primary CD4+ T cells after knock-out of DNAJC9 by CRISPR/CAS9.
 |
Sven (Verified Customer) (11-27-2021) | Works very well on western blot for human and mouse tissue (1:5000 dilution in TBS-T with 50% glycerol, 2% BSA, 0.02% NaN3). Antiboydy specificity was validated by DNAJC9 knockout cells
 |
