Tested Applications
| Positive WB detected in | human urine sample |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16659-1-AP targets DNase I in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10117 Product name: Recombinant human DNASE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC029437 Sequence: MRGMKLLGALLALAALLQGAVSLKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHYPVEVMLK Predict reactive species |
| Full Name | deoxyribonuclease I |
| Calculated Molecular Weight | 282 aa, 31 kDa |
| Observed Molecular Weight | 31-40 kDa |
| GenBank Accession Number | BC029437 |
| Gene Symbol | DNASE1 |
| Gene ID (NCBI) | 1773 |
| RRID | AB_3669253 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24855 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DNase I antibody 16659-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNase I Polyclonal antibody (Cat# 16659-1-AP) has 1 total citation. It appears in ACS Nano (IF 17.3), in a study on nano-enabled zooremediation of antibiotic resistance genes via interfacial DNA capture and stimulated nuclease secretion. The research field encompasses nanotechnology, environmental bioremediation, and nucleic acid metabolism. The antibody was applied in WB and IHC in this publication.
| Species | Application | Title |
|---|---|---|
ACS Nano Nano-Enabled Zooremediation of Antibiotic Resistance Genes via Interfacial DNA Capture and Stimulated Nuclease Secretion. |
