Dynamin 1 Polyclonal antibody

Dynamin 1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18205-1-AP

Synonyms

DNM, DNM1, dynamin 1, Dynamin1



Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

18205-1-AP targets Dynamin 1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dynamin 1 fusion protein Ag12827
Full Name dynamin 1
Calculated molecular weight 851 aa, 96 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC063850
Gene symbol DNM1
Gene ID (NCBI) 1759
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Dynamin 1 antibody 18205-1-APDownload protocol
IHC protocol for Dynamin 1 antibody 18205-1-APDownload protocol
IF protocol for Dynamin 1 antibody 18205-1-APDownload protocol
IP protocol for Dynamin 1 antibody 18205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

J Trace Elem Med Biol

Cu-induced mitochondrial dysfunction is mediated by abnormal mitochondrial fission through oxidative stress in primary chicken embryo hepatocytes.

Authors - Liuyan Wu
humanWB

Neurobiol Dis

Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.

Authors - Li Zou
humanWB

Elife

Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.

Authors - Xingyu Zhang