Dynamin 1 Polyclonal antibody

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• Dynamin 1 Polyclonal antibody (18205-1-AP)

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• KD/KO Validated

Dynamin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18205-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DNM, DNM1, dynamin 1, Dynamin1

• 

  WB analysis of mouse brain using 18205-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 18205-1-AP

  WB result of Dynamin 1 antibody (18205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Dynamin 1 transfected A549 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 18205-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 18205-1-AP

  IP result of anti-Dynamin 1 (IP:18205-1-AP, 4ug; Detection:18205-1-AP 1:2000) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Neuro-2a using 18205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using 18205-1-AP (Dynamin 1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, NIH/3T3 cells, A549 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18205-1-AP targets Dynamin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig, chicken, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12827

  Product name: Recombinant human DNM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 502-851 aa of BC063850

  Sequence: RSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAPPGVPRITISDP

• Full Name: dynamin 1
• Calculated Molecular Weight: 851 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC063850
• Gene Symbol: DNM1
• Gene ID (NCBI): 1759
• RRID: AB_2093197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05193
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Dynamin 1 Polyclonal antibody (Cat# 18205-1-AP) has 10 citations published in journals including Neuron, PNAS, Neurobiology of Disease, Communications Biology, International Journal of Molecular Sciences, PLoS Pathogens, Journal of Neuroscience, Journal of Trace Elements in Medicine and Biology, Virus Research, and eLife. Research fields span neuroscience (synaptic dysfunction, sleep regulation, Parkinson's disease), oncology (lung cancer chemoresistance), virology (SARS-CoV-2, pseudorabies virus), mitochondrial biology, and lipid metabolism.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Neuron; Neurons undergo IFNγ-driven persistent epigenetic shifts and synaptopathy in encephalitis.; Authors - Ghazal Shammas
mouse	WB	Proc Natl Acad Sci U S A; Calcineurin governs baseline and homeostatic regulations of non-rapid eye movement sleep in mice; Authors - Xin Yin
human	WB	Neurobiol Dis; Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.; Authors - Li Zou
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
fish	WB	Int J Mol Sci; Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish.; Authors - Shu-Wei Chen
mouse,pig	WB	PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su