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DNMT1 Antibody

DNMT1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (5)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 24206-1-AP

Synonyms

AIM, CXXC9, DNA MTase HsaI, DNMT, DNMT1, MCMT



Tested Applications

Positive WB detected inHEK-293 cells, C6 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The 24206-1-AP immunogen is Fusion Protein DNMT1 protein expressed in E. coli.

Reactivity human, rat
Cited Species Chicken, gilt, hen, hens, human, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT1 fusion protein Ag19116
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated molecular weight 1632aa,185 kDa
Observed molecular weight 180-200 kDa
GenBank accession number BC126227
Gene symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MV 184 kDa and 145 kDa. (PMID: 10753866)

Protocols

Product Specific Protocols
WB protocol for DNMT1 antibody 24206-1-APDownload protocol
IF protocol for DNMT1 antibody 24206-1-APDownload protocol
IP protocol for DNMT1 antibody 24206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Anim Sci Biotechnol

Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.

Authors - Nagmeldin A Omer
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
chickenWB

Comp Biochem Physiol B Biochem Mol Biol

Maternal betaine administration modulates hepatic type 1 iodothyronine deiodinase (Dio1) expression in chicken offspring through epigenetic modifications.

Authors - Zhen Hou
ChickenWB

Comp Biochem Physiol B Biochem Mol Biol

Dietary betaine supplementation in hens modulates hypothalamic expression of cholesterol metabolic genes in F1 cockerels through modification of DNA methylation.

Authors - Abdulrahman A Idriss
humanWB

Front Oncol

Small Molecule Inhibitor C188-9 Synergistically Enhances the Demethylated Activity of Low-Dose 5-Aza-2'-Deoxycytidine Against Pancreatic Cancer.

Authors - Rui Kong
humanWB

Poult Sci

Prenatal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in cockerels through modifications of DNA methylation.

Authors - Abdulrahman A Idriss