• Featured Product
  • KD/KO Validated

DNMT1 Polyclonal antibody

DNMT1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (5)





Cat no : 24206-1-AP


AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, MCMT

Tested Applications

Positive WB detected inHeLa cells, C6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24206-1-AP targets DNMT1 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT1 fusion protein Ag19116
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated Molecular Weight 1632 aa, 185 kDa
Observed Molecular Weight180-200 kDa
GenBank Accession NumberBC126227
Gene Symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)


Product Specific Protocols
WB protocol for DNMT1 antibody 24206-1-APDownload protocol
IHC protocol for DNMT1 antibody 24206-1-APDownload protocol
IF protocol for DNMT1 antibody 24206-1-APDownload protocol
IP protocol for DNMT1 antibody 24206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Dis

Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Authors - Bowen Liu


Cyhexatin causes developmental toxic effects by disrupting endocrine system and inducing behavioral inhibition, apoptosis and DNA hypomethylation in zebrafish (Danio rerio) larvae

Authors - Fang Jiao

Bioact Mater

Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.

Authors - Jin Wang
  • KO Validated

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou

J Transl Med

The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy

Authors - Liangliang Zhao

Respir Res

Oxidative stress mediates the apoptosis and epigenetic modification of the Bcl-2 promoter via DNMT1 in a cigarette smoke-induced emphysema model.

Authors - Huihui Zeng


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Paula (Verified Customer) (12-14-2021)

good bands at the right molecular weight, but a lot of smear

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: fetal rat lung tissue
DNMT1 Antibody Western Blot validation (1:500 dilution) in fetal rat lung tissue (Cat no:24206-1-AP)