DNMT2 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• DNMT2 Polyclonal antibody, PBS Only (19221-1-PBS)

• 

  WB analysis of HeLa using 19221-1-AP (same clone as 19221-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 19221-1-AP (same clone as 19221-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 19221-1-AP (same clone as 19221-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 19221-1-AP (same clone as 19221-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19221-1-AP (same clone as 19221-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19221-1-AP (DNMT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19221-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DNMT2 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 19221-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

TRDMT1, cytosine-5, DNA, DNA MTase homolog HsaIIP, EC:2.1.1.204

---

Product Information

19221-1-PBS targets DNMT2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5571

  Product name: Recombinant human DNMT2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-391 aa of BC047733

  Sequence: MEPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRTNSFLYILDILPRLQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKILYE

• Full Name: tRNA aspartic acid methyltransferase 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC047733
• Gene Symbol: DNMT2
• Gene ID (NCBI): 1787
• RRID: AB_10597075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14717
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Dnmt2 was initially assigned a member of the DNA methyltransferase family on the basis of its extensive homology with eukaryotic and prokaryotic DNA-(cytosine C5)-methyltransferases . However, in the apparent absence of a phenotype in dnmt2 knockout cells, Dnmt2's possible biological function remained unknown , even though Dnmt2 is strongly conserved and it is found in species ranging from Schizosaccharomyces pombe to human. Later very weak, residual DNA methylation activity was found with enzymes from different species. The finding that Dnmt2 is an active RNA methyltransferase capable of methylating the C38 position of the tRNA Asp came as a surprise . However, still no cellular function of the tRNA Asp methylation has been found, although in Zebrafish Dnmt2 knock-down caused a developmental phenotype . It is very intriguing that an enzyme that looks like a DNA methyltransferase can methylate RNA, in particular since the RNA and DNA specific m5 C methyltransferases use different catalytic residues and a different mechanism for the methyl transfer reaction . the predicted molecular weight of Dnmt2 is 45 kDa, but it may migrate to the 40 kDa (PMID: 18567810 )