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- KD/KO Validated
DNMT3A Polyclonal antibody
DNMT3A Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, RIP, IP, IHC, IF, ChIP, ELISA
Cat no : 20954-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cels, mouse testis tissue, rat testis tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human stomach cancer tissue, mouse colon tissue, mouse testis tissue, rat colon tissue, rat testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||K-562 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 22 publications below|
|IHC||See 1 publications below|
|IF||See 2 publications below|
|ChIP||See 3 publications below|
|RIP||See 1 publications below|
The immunogen of 20954-1-AP is DNMT3A Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNMT3A fusion protein Ag15111|
|Full Name||DNA (cytosine-5-)-methyltransferase 3 alpha|
|Calculated molecular weight||912 aa, 102 kDa|
|Observed molecular weight||120-130 kDa|
|GenBank accession number||BC043617|
|Gene ID (NCBI)||1788|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.
|Product Specific Protocols|
|WB protocol for DNMT3A antibody 20954-1-AP||Download protocol|
|IHC protocol for DNMT3A antibody 20954-1-AP||Download protocol|
|IF protocol for DNMT3A antibody 20954-1-AP||Download protocol|
|IP protocol for DNMT3A antibody 20954-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CircNTNG1 inhibits renal cell carcinoma progression via HOXA5-mediated epigenetic silencing of Slug
Tumor suppressor CEBPA interacts with and inhibits DNMT3A activity.
Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.
Cell Biol Toxicol
Hypermethylation of microRNA-497-3p contributes to progression of thyroid cancer through activation of PAK1/β-catenin.
J Mol Cell Cardiol
LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.
Front Cell Dev Biol
piRNA-823 Is Involved in Cancer Stem Cell Regulation Through Altering DNA Methylation in Association With Luminal Breast Cancer.