DNMT3A Antibody

DNMT3A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20954-1-AP

Synonyms

DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT3A, DNMT3A2, M.HsaIIIA



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse testis tissue, NIH/3T3 cells, PC-3 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

The 20954-1-AP immunogen is Fusion Protein DNMT3A protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT3A fusion protein Ag15111
Full Name DNA (cytosine-5-)-methyltransferase 3 alpha
Calculated molecular weight 912aa,102 kDa
Observed molecular weight 120-130 kDa
GenBank accession number BC043617
Gene symbol DNMT3A
Gene ID (NCBI) 1788
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.

Protocols

Product Specific Protocols
WB protocol for DNMT3A antibody 20954-1-APDownload protocol
IP protocol for DNMT3A antibody 20954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biol Int

miR-194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts.

Authors - Shun Xu
humanWB

Life Sci

Upregulation of programmed death ligand 1 by liver kinase B1 and its implication in programmed death 1 blockade therapy in non-small cell lung cancer.

Authors - Xiangfeng Shen
mouseWB

Epigenetics Chromatin

Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice.

Authors - Jordan M Buck
humanWB

J Mol Cell Cardiol

LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.

Authors - Xinxing Zhu