DNMT3A Recombinant antibody

• 

  IHC staining of human placenta using 81474-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81474-6-RR (DNMT3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 81474-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNMT3A antibody (81474-6-RR, Clone: 242257A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 81474-6-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Dnmt3a Recombinant Antibody (81474-6-RR, Clone:242257A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 81474-6-RR

  Biolayer interferometry (BLl) kinetic assays of 81474-6-RR against Human DNMT3A were performed. The affinity constant is 5.22 nM.

DNMT3A Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 81474-6-RR

Clone No.242257A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

242257A9, Cysteine methyltransferase DNMT3A, DNA (cytosine-5)-methyltransferase 3A, DNA MTase HsaIIIA, EC:2.1.1.-

---

Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81474-6-RR targets DNMT3A in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DNMT3A fusion protein Ag15111
• Full Name: DNA (cytosine-5-)-methyltransferase 3 alpha
• Calculated Molecular Weight: 912 aa, 102 kDa
• GenBank Accession Number: BC043617
• Gene Symbol: DNMT3A
• Gene ID (NCBI): 1788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y6K1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a.