DNMT3B Polyclonal antibody

DNMT3B Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26971-1-AP

Synonyms

DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, DNMT3B, ICF, M.HsaIIIB



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26971-1-AP is DNMT3B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity goat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT3B fusion protein Ag25117
Full Name DNA (cytosine-5-)-methyltransferase 3 beta
Calculated molecular weight 95 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberNM_001207055
Gene symbol DNMT3B
Gene ID (NCBI) 1789
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNMT3B antibody 26971-1-APDownload protocol
IHC protocol for DNMT3B antibody 26971-1-APDownload protocol
IF protocol for DNMT3B antibody 26971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
goatWB,IF

Res Vet Sci

Epigenetic modification differences between fetal fibroblast cells and mesenchymal stem cells of the Arbas Cashmere goat.

Authors - Xiao Wang
humanWB

Brain Sci

Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes.

Authors - Kalaiselvi Sivalingam