DNMT3L Polyclonal antibody

• 

  WB analysis of PC-3 using 14939-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14939-1-AP (DNMT3L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14939-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14939-1-AP (DNMT3L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

DNMT3L Polyclonal Antibody for WB, ELISA

Cat No. 14939-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DNA (cytosine-5)-methyltransferase 3-like

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Tested Applications

• Positive WB detected in: mouse testis tissue, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14939-1-AP targets DNMT3L in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6822

  Product name: Recombinant human DNMT3L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-386 aa of BC002560

  Sequence: MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGRDLIAYEVKANQRNIEDICICCGSLQVHTQHPLFEGGICAPCKDKFLDALFLYDDDGYQSYCSICCSGETLLICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNWVCYLCLPSSRSGLLQRRRKWRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPGPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL

• Full Name: DNA (cytosine-5-)-methyltransferase 3-like
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa and 70 kDa
• GenBank Accession Number: BC002560
• Gene Symbol: DNMT3L
• Gene ID (NCBI): 29947
• RRID: AB_2094281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJW3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	J Reprod Immunol; Decreased expression and DNA hypermethylation of syncytin-1 in human villus tissues with unexplained recurrent spontaneous abortion; Authors - Yang Fu