DNPEP Polyclonal antibody

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  WB analysis using 15016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15016-1-AP (DNPEP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15016-1-AP

  WB result of DNPEP antibody (15016-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNPEP transfected MCF-7 cells.

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  IP experiment of HEK-293 using 15016-1-AP

  IP result of anti-DNPEP (IP:15016-1-AP, 3ug; Detection:15016-1-AP 1:500) with HEK-293 cells lysate 1760ug.

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  IHC staining of mouse testis using 15016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15016-1-AP (DNPEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15016-1-AP (DNPEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 15016-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DNPEP antibody (15016-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

DNPEP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 15016-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

EC:3.4.11.21, DAP, ASPEP

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15016-1-AP targets DNPEP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6966

  Product name: Recombinant human DNPEP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 130-475 aa of BC000653

  Sequence: FDRDLTLAGRVIVKCPTSGRLEQQLVHVERPILRIPHLAIHLQRNINENFGPNTEMHLVPILATAIQEELEKGTPEPGPLNAVDERHHSVLMSLLCAHLGLSPKDIVEMELCLADTQPAVLGGAYDEFIFAPRLDNLHSCFCALQALIDSCAGPGSLATEPHVRMVTLYDNEEVGSESAQGAQSLLTELVLRRISASCQHPTAFEEAIPKSFMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNAVSEALIREVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLRVLDLGSPQLAMHSIREMACTTGVLQTLTLFKGFFELFPSLSHNLLVD

• Full Name: aspartyl aminopeptidase
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC000653
• Gene Symbol: DNPEP
• Gene ID (NCBI): 23549
• RRID: AB_2094293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULA0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNPEP, also named as ASPEP and DAP, plays an important role in intracellular protein and peptide metabolism. It catalyzes the hydrolysis of N-terminal aspartyl and glutamyl residues.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Cell Biol; Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate bone morphogenetic protein signaling.; Authors - Nakamura Yukio Y