DNTT,TDT Polyclonal antibody

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  IP experiment of Raji using 17382-1-AP

  IP result of anti-DNTT,TDT (IP:17382-1-AP, 4ug; Detection:17382-1-AP 1:300) with Raji cells lysate 2000ug.

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  IHC staining of human lymphoma using 17382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 17382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 17382-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DNTT,TDT Polyclonal antibody (17382-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

DNTT,TDT Polyclonal Antibody for IHC, FC (Intra), IP, ELISA

Cat No. 17382-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra), IP, ELISA

Synonyms

DNTT, DNA nucleotidylexotransferase, EC:2.7.7.31, TDT, Terminal Deoxynucleotidyl Transferase

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Tested Applications

• Positive IP detected in: Raji cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17382-1-AP targets DNTT,TDT in IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11235

  Product name: Recombinant human DNTT,TDT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 162-509 aa of BC012920

  Sequence: NNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA

• Full Name: deoxynucleotidyltransferase, terminal
• Calculated Molecular Weight: 509 aa, 58 kDa
• GenBank Accession Number: BC012920
• Gene Symbol: DNTT
• Gene ID (NCBI): 1791
• RRID: AB_2878399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04053
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.