DNTT,TDT Polyclonal antibody

DNTT,TDT Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17382-1-AP

Synonyms

DNA nucleotidylexotransferase, DNTT, TDT, Terminal addition enzyme, Terminal transferase

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Validation Data Gallery

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  IP experiment of Raji using 17382-1-AP

  IP result of anti-DNTT,TDT (IP:17382-1-AP, 4ug; Detection:17382-1-AP 1:300) with Raji cells lysate 2000ug.

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  IHC staining of human lymphoma using 17382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 17382-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 17382-1-AP (DNTT,TDT antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IP detected in: Raji cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17382-1-AP is DNTT,TDT Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNTT,TDT fusion protein Ag11235
• Full Name: deoxynucleotidyltransferase, terminal
• Calculated Molecular Weight: 509 aa, 58 kDa
• GenBank Accession Number: BC012920
• Gene Symbol: DNTT
• Gene ID (NCBI): 1791
• RRID: AB_2878399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.