DNTT,TDT Polyclonal antibody

DNTT,TDT Polyclonal Antibody for FC, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 17382-1-AP

Synonyms

DNA nucleotidylexotransferase, DNTT, TDT, Terminal addition enzyme, Terminal transferase



Tested Applications

Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17382-1-AP is DNTT,TDT Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNTT,TDT fusion protein Ag11235
Full Name deoxynucleotidyltransferase, terminal
Calculated molecular weight 509 aa, 58 kDa
GenBank accession numberBC012920
Gene symbol DNTT
Gene ID (NCBI) 1791
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for DNTT,TDT antibody 17382-1-APDownload protocol
IP protocol for DNTT,TDT antibody 17382-1-APDownload protocol
FC protocol for DNTT,TDT antibody 17382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols