DOCK2 Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 66969-1-Ig (same clone as 66969-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 66969-1-Ig (same clone as 66969-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66969-1-Ig (DOCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66969-1-Ig (DOCK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66969-1-Ig (same clone as 66969-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK2 antibody (66969-1-Ig, Clone: 1D7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66969-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DOCK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66969-1-PBS

Clone No.1D7A8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209

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Product Information

66969-1-PBS targets DOCK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28582

  Product name: Recombinant human DOCK2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-337 aa of BC016996

  Sequence: MCVCAEDGKTLPVAVPIEDMQRIHLRFMFRHRSSLESKDKGEKNFAMSYVKLMKEDGTTLHDGFHDLVVLKGDSKKMEDASAYLTLPSYRHHVENKGATLSRSSSSVGGLSVSSRDVFSISTLVCSTKLTQNVGLLGLLKWRMKPQLEESMRRLFESINNLMKSQYKTTILLQVAALKYIPSVLHDVEMVFDAKLLSQLLYEFYTCIPPVKLQKQKVQSMNEIVQSNLFKKQECRDILLPVITKELKELLEQKDDMQHQVLERKYCVELLNSILEVLSYQDAAFTYHHIQEIMVQLLRTVNRTVITMGRDHILIGACCNCGFPSSTPHSLIQWLWG

• Full Name: dedicator of cytokinesis 2
• Calculated Molecular Weight: 212 kDa, 38 kDa
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC016996
• Gene Symbol: DOCK2
• Gene ID (NCBI): 1794
• RRID: AB_2882291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q92608
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.