Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T cells |
| Positive IP detected in | HeLa cells, HEK-293T cells |
| Positive IHC detected in | mouse ovary tissue, human prostate cancer tissue, human ovary tumor tissue, human breast cancer tissue, human placenta tissue, human rectal cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:150-1:600 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21861-1-AP targets DOCK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16516 Product name: Recombinant human DOCK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1703-2011 aa of BC117689 Sequence: TGQSESSDEVFNMQPSPSTSSLSSTHSASPNVTSSAPSSARASPLLSDKHKHSRENSCLSPRERPCSAIYPTPVEPSQRMLFNHIGDGALPRSDPNLSAPEKAVNPTPSSWSLDSGKEAKNMSDSGKLISPPVPPRPTQTASPARHTTSVSPSPAGRSPLKGSVQSFTPSPVEYHSPGLISNSPVLSGSYSSGISSLSRCSTSETSGFENQVNEQSAPLPVPVPVPVPSYGGEEPVRKESKTPPPYSVYERTLRRPIPLPHSLSIPVTSEPPALPPKPLAARSSHLENGARRTDPGPRPRPLPRKVSQL Predict reactive species |
| Full Name | dedicator of cytokinesis 4 |
| Calculated Molecular Weight | 2011 aa, 230 kDa |
| Observed Molecular Weight | 225 kDa |
| GenBank Accession Number | BC117689 |
| Gene Symbol | DOCK4 |
| Gene ID (NCBI) | 9732 |
| RRID | AB_2878930 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N1I0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DOCK4 antibody 21861-1-AP | Download protocol |
| IHC protocol for DOCK4 antibody 21861-1-AP | Download protocol |
| IP protocol for DOCK4 antibody 21861-1-AP | Download protocol |
| WB protocol for DOCK4 antibody 21861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DOCK4 antibody (Cat# 21861-1-AP) has 6 citations published in Science, PNAS, Cell Oncology, Frontiers in Cell and Developmental Biology, Annals of Medicine, and Frontiers in Oncology. Research fields span cell surface proximity labeling, placenta accreta spectrum, ovarian cancer metastasis via Rap1 signaling, diabetic renal tubular injury through Wnt/β-catenin pathway, clear cell renal cell carcinoma prognosis, and prostate cancer tumor suppression. Applications include WB, IF, IP, and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Science Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells | ||
Proc Natl Acad Sci U S A Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum. | ||
Cell Oncol (Dordr) THEMIS2 contributes to ovarian cancer metastasis via DOCK4-mediated activation of Rap1 signaling | ||
Front Cell Dev Biol USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway. | ||
Ann Med Elevated DOCK4 expression correlates with favorable prognosis and immune infiltration in clear cell renal cell carcinoma. | ||
