DOCK6 Polyclonal antibody

DOCK6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25087-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

dedicator of cytokinesis 6, ZIR1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 25087-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 25087-1-AP (DOCK6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 25087-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 25087-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 25087-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 25087-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat colon using 25087-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25087-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25087-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25087-1-AP (DOCK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	COLO 320 cells
Positive IHC detected in	mouse colon tissue, human rectal cancer tissue, rat colon tissue, human colon cancer tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25087-1-AP targets DOCK6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18901 Product name: Recombinant human DOCK6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1699-2041 aa of BC146786 Sequence: LYEAVNEVYKNLIPILEAHRDYKKLAAVHGKLQEAFTKIMHQSSGWERVFGTYFRVGFYGAHFGDLDEQEFVYKEPSITKLAEISHRLEEFYTERFGDDVVEIIKDSNPVDKSKLDSQKAYIQITYVEPYFDTYELKDRVTYFDRNYGLRTFLFCTPFTPDGRAHGELPEQHKRKTLLSTDHAFPYIKTRIRVCHREETVLTPVEVAIEDMQKKTRELAFATEQDPPDAKMLQMVLQGSVGPTVNQGPLEVAQVFLAEIPEDPKLFRHHNKLRLCFKDFCKKCEDALRKNKALIGPDQKEYHRELERNYCRLREALQPLLTQRLPQLMAPTPPGLRNSLNRAS Predict reactive species
Full Name	dedicator of cytokinesis 6
Calculated Molecular Weight	2047 aa, 230 kDa
Observed Molecular Weight	230 kDa
GenBank Accession Number	BC146786
Gene Symbol	DOCK6
Gene ID (NCBI)	57572
RRID	AB_2879889
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96HP0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for DOCK6 antibody 25087-1-AP	Download protocol
WB protocol for DOCK6 antibody 25087-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The DOCK6 Polyclonal antibody (Cat# 25087-1-AP) has 2 total citations. One appears in Nature Cell Biology (2020), studying single-cell reconstruction of the adult human heart during heart failure and recovery. The other is in BMC Medical Genomics (2022), investigating attenuated clinical and osteoclastic phenotypes of Paget's disease of bone linked to a rare variant in the DOCK6 gene. The associated research fields are cardiac biology and bone genetics.

Species	Application	Title
human	IF	Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. Authors - Li Wang View Article
human	WB	BMC Med Genomics Attenuated clinical and osteoclastic phenotypes of Paget's disease of bone linked to the p.Pro392Leu/SQSTM1 mutation by a rare variant in the DOCK6 gene. Authors - Mariam Dessay View Article