DOK3 Polyclonal antibody

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  WB analysis using 15749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15749-1-AP (DOK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

DOK3 Polyclonal Antibody for WB, ELISA

Cat No. 15749-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

docking protein 3, DOK3, DOKL

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Tested Applications

• Positive WB detected in: K-562 cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

15749-1-AP targets DOK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOK3 fusion protein Ag8221
• Full Name: docking protein 3
• Calculated Molecular Weight: 496aa,53 kDa; 330aa,36 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC004564
• Gene Symbol: DOK3
• Gene ID (NCBI): 79930
• RRID: AB_2095099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L591
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The adaptor proteins downstream of kinase 1 (DOK1) to DOK3 are mainly found in immune cells, and they are closely related, with some functional redundancy, although each DOK protein has distinct properties and functions. DOK1 to DOK3 inhibit LPS responses. DOK proteins have an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and four tyrosine-containing motifs that can bind to Src homology 2 (SH2) domains when phosphorylated. DOK3 was also shown to have an inhibitory role as it restricts Ca2+ and JNK activation in B cell receptor signaling. The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.

Publications

Species	Application	Title
human	IHC	Int Immunopharmacol; Elevated expression of DOK3 indicates high suppressive immune cell infiltration and unfavorable prognosis of gliomas.; Authors - Xiu Liu