DOK4 Polyclonal antibody

DOK4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 10481-2-AP

Synonyms

docking protein 4, DOK4, IRS 5, IRS5

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Validation Data Gallery

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  IHC staining of human prostate cancer using 10481-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10481-2-AP (DOK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10481-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10481-2-AP (DOK4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

10481-2-AP targets DOK4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOK4 fusion protein Ag0734
• Full Name: docking protein 4
• Calculated molecular weight: 37 kDa
• GenBank accession number: BC003541
• Gene symbol: DOK4
• Gene ID (NCBI): 55715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The INS-receptor substrate family plays important roles in cellular growth, signaling and survival. IRS5/DOK4 is a new member of this family identified, which is known as docking protein 4, or downstream of tyrosine kinase-4. DOK4 might have roles in INS and IGF-1 signaling as well. Expression of IRS5/DOK4 is regulated epigenetically via histone acetylating at its promoter. DOK4 is required at early stages in the myelination process, including the initial interaction with the axon, and is also involved in Schwann cell migration and proliferation, it also regulates GDNF-mediated neurite outgrowth during neuronal development through activation of the Rap1-ERK1/2 pathway. DOK4 may represent a novel negative regulator of T cells as other DOK family members like DOK1 and DOK2.