DOM3Z Polyclonal antibody

• Featured Product
• KD/KO Validated

DOM3Z Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11015-2-AP

Synonyms

DXO, Dom-3 homolog Z, DOM 3Z, DeNADding enzyme DXO, Decapping and exoribonuclease protein

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Validation Data Gallery

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  WB analysis of mouse testis using 11015-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11015-2-AP (DOM3Z antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11015-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11015-2-AP (DOM3Z antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11015-2-AP

  IP result of anti-DOM3Z (IP:11015-2-AP, 4ug; Detection:11015-2-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 11015-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11015-2-AP (DOM3Z antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human breast cancer using 11015-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11015-2-AP (DOM3Z antibody) at dilution of 1:50 (under 25x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, SH-SY5Y cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

11015-2-AP targets DOM3Z in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOM3Z fusion protein Ag1491
• Full Name: dom-3 homolog Z (C. elegans)
• Calculated Molecular Weight: 43.6 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC009344
• Gene Symbol: DOM3Z
• Gene ID (NCBI): 1797
• RRID: AB_2095257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOM3Z also known as DXO, NG6, is a 396 amino acid ubiquitously expressed protein. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6 and possesses RNA 5'-pyrophosphohydrolase activity. Chromosome 6 contains 170 million base pairs and represents nearly 6% of the human genome. Partial deletion of the q arm of chromosome 6 is associated with early-onset colorectal cancer. DOM3Z is ubiquitously expressed, with high expression in the testis (PMID: 9799600).

Publications

Species	Application	Title
human	WB	Cell; 5' End Nicotinamide Adenine Dinucleotide Cap in Human Cells Promotes RNA Decay through DXO-Mediated deNADding.; Authors - Xinfu Jiao ; KO Validated
human	WB	Mol Cell; A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.; Authors - Xinfu Jiao ; KD Validated
human	WB,IF	RNA; Unraveling regulation and new components of human P-bodies through a protein interaction framework and experimental validation.; Authors - Zheng Dinghai D
human	IF	PLoS One; 2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.; Authors - Frédéric Picard-Jean
human	WB	Nat Chem Biol; Structural and mechanistic basis of mammalian Nudt12 RNA deNADding.; Authors - Ewa Grudzien-Nogalska ; KO Validated