DOPA decarboxylase/DDC Recombinant antibody

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  WB analysis using 85811-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85811-2-RR (DOPA decarboxylase/DDC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DOPA decarboxylase/DDC Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85811-2-RR

Clone No.243125A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85811-2-RR targets DOPA decarboxylase/DDC in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DOPA decarboxylase/DDC fusion protein Ag0219
• Full Name: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC008366
• Gene Symbol: DOPA decarboxylase
• Gene ID (NCBI): 1644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20711
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily. DDC is an enzyme that converts levodopa into dopamine14, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 3374198, PMID: 28100251). DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.