Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, Y79 cells, HepG2 cells, mouse thymus tissue, K-562 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lung cancer tissue, human testis tissue, human pituitary adenoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12111-1-AP targets DPF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2756 Product name: Recombinant human DPF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC014889 Sequence: MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKRHRGPGLASGQLYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLEALLRTDPLEKRGAPDPRVDDDSLGEFPVTNSRARKRILEPDDFLDDLDDEDYEEDTPKRRGKGKSKGKGVGSARKKLDASILEDRDKPYACDICGKRYKNRPGLSYHYAHSHLAEEEGEDKEDSQPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDSKINKKTGQPEELVSCSDCGRSGHPSCLQFTPVMMAAVKTYRWQCIECKCCNICGTSENDDQLLFCDDCDRGYHMYCLTPSMSEPPEGSWSCHLCLDLLKEKASIYQNQNSS Predict reactive species |
| Full Name | D4, zinc and double PHD fingers family 2 |
| Calculated Molecular Weight | 391 aa, 44 kDa |
| Observed Molecular Weight | 44-50 kDa |
| GenBank Accession Number | BC014889 |
| Gene Symbol | DPF2 |
| Gene ID (NCBI) | 5977 |
| RRID | AB_2095574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92785 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DPF2, also named as BAF45D or UBID4, is a 391 amino acid protein, which belongs to the requiem/DPF family. DPF2 may be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells and might also have a role in the development and maturation of lymphoid cells. DPF2 was recently shown to inhibit the myeloid differentiation of hematopoietic stem/progenitor and acute myelogenous leukemia cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DPF2 antibody 12111-1-AP | Download protocol |
| IHC protocol for DPF2 antibody 12111-1-AP | Download protocol |
| IP protocol for DPF2 antibody 12111-1-AP | Download protocol |
| WB protocol for DPF2 antibody 12111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DPF2 (BAF45D) polyclonal antibody (Cat# 12111-1-AP) has 11 citations spanning journals including Nature Communications, Molecular Cell, Science China Life Sciences, Analytical Chemistry, International Journal of Molecular Sciences, Frontiers in Neuroscience, Journal of Cancer, European Journal of Neuroscience, Frontiers in Neuroanatomy, Bioscience Biotechnology and Biochemistry, and Biochimica et Biophysica Acta. Research fields cover chromatin biology, cancer, neuroscience, stem cell biology, and inflammation.
| Species | Application | Title |
|---|---|---|
Sci China Life Sci Inhibition of recurrence and metastasis in triple-negative breast cancer through nanoparticle-mediated silencing of LPCAT1 to remodel ATP energy metabolism | ||
Anal Chem Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling. | ||
Int J Mol Sci A High-Calorie Diet Aggravates Lipopolysaccharide-Induced Pulmonary Inflammation in Juvenile Rats via Hypothalamic-Pituitary-Adrenal Axis-Related Pathways | ||
Front Neurosci BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions.
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