DPH2 Polyclonal antibody, PBS Only

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• DPH2 Polyclonal antibody, PBS Only (12367-1-PBS)

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  WB analysis of human brain using 12367-1-AP (same clone as 12367-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  WB analysis using 12367-1-AP (same clone as 12367-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12367-1-AP (DPH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 12367-1-AP (same clone as 12367-1-PBS)

  IP result of anti-DPH2 (IP:12367-1-AP, 3ug; Detection:12367-1-AP 1:1000) with mouse skeletal muscle tissue lysate 6000ug. This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12367-1-AP (same clone as 12367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12367-1-AP (DPH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 12367-1-AP (same clone as 12367-1-PBS)

  Immunofluorescent analysis of PC-3 cells using 12367-1-AP (DPH2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12367-1-PBS in a different storage buffer formulation.

DPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12367-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

12367-1-PBS targets DPH2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3042

  Product name: Recombinant human DPH2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 190-489 aa of BC016956

  Sequence: SPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG

• Full Name: DPH2 homolog (S. cerevisiae)
• Calculated Molecular Weight: 489 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC016956
• Gene Symbol: DPH2
• Gene ID (NCBI): 1802
• RRID: AB_2093256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQC3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Mutants of the DPH1, DPH2, and DPH5 (611075) genes, involved in diphthamide biosynthesis, were identified as resistant to both anthrax toxin and diphtheria toxin. This antibody works well in WB, IP, IHC and IF application.