DPM1 Polyclonal antibody

DPM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12403-2-AP

Synonyms

CDGIE, DPM synthase, DPM1, Mannose P dolichol synthase, MPD synthase, MPDS

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Validation Data Gallery

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  WB analysis of HeLa using 12403-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12403-2-AP (DPM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12403-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12403-2-AP (DPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12403-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12403-2-AP (DPM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12403-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12403-2-AP (DPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12403-2-AP targets DPM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DPM1 fusion protein Ag1128
• Full Name: dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
• Calculated Molecular Weight: 260 aa, 30 kDa
• Observed Molecular Weight: 27-32 kDa
• GenBank Accession Number: BC007073
• Gene Symbol: DPM1
• Gene ID (NCBI): 8813
• RRID: AB_2093270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Genet; Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm.; Authors - Zhiyang Zhou
	WB	FEBS Lett; Aspirin damages the cell wall of Saccharomyces cerevisiae by inhibiting the expression and activity of dolichol-phosphate mannose synthase 1.; Authors - Ming Li