Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue, rat lung tissue |
| Positive IHC detected in | rat liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
32397-1-AP targets DPP4/CD26 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | dipeptidylpeptidase 4 |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | NM_010074.3 |
| Gene Symbol | Dpp4 |
| Gene ID (NCBI) | 13482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P28843 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DPP4/CD26 antibody 32397-1-AP | Download protocol |
| WB protocol for DPP4/CD26 antibody 32397-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
