DPP4/CD26 Monoclonal antibody

DPP4/CD26 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rabbit

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2G6D11

Cat no : 68383-1-Ig

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

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Validation Data Gallery

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  WB analysis of human placenta using 68383-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig kidney using 68383-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit kidney using 68383-1-Ig

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 68383-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HuH-7 using 68383-1-Ig

  1X10^6 HuH-7 cells were surface stained with 0.4 ug Anti-Human DPP4/CD26 (68383-1-Ig, Clone:2G6D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed .

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Tested Applications

• Positive WB detected in: human placenta tissue, HuH-7 cells, pig kidney tissue, rabbit kidney tissue
• Positive FC detected in: HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68383-1-Ig targets DPP4/CD26 in WB, FC, ELISA applications and shows reactivity with Human, Pig, Rabbit samples.

• Tested Reactivity: Human, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: dipeptidyl-peptidase 4
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC013329
• Gene Symbol: DPP4
• Gene ID (NCBI): 1803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).