DPPA3 Polyclonal antibody

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• DPPA3 Polyclonal antibody (33565-1-AP)

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  WB analysis using 33565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33565-1-AP (DPPA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

DPPA3 Polyclonal Antibody for WB, ELISA

Cat No. 33565-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Developmental pluripotency-associated protein 3, STELLAR, Stella-related protein

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Tested Applications

• Positive WB detected in: human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33565-1-AP targets DPPA3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34680

  Product name: Recombinant human DPPA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-159 aa of BC160067

  Sequence: MDPSQFNPTYIPGSPQMLTEENSRDDSGASQISSETLIKNLSNLTINASSESVSPLSEALLRRESVGAAVLREIEDEWLYSRRGVRTLLSVQREKMARLRYMLLGGVRTHERRPTNKEPKGVKKESRPFKCPCSFCVSNGWDPSENARIGNQDTKPLQP

• Full Name: developmental pluripotency associated 3
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC160067
• Gene Symbol: DPPA3
• Gene ID (NCBI): 359787
• RRID: AB_3743011
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6W0C5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPPA3 (developmental pluripotency related 3), also known as STELLAR, which is a protein which plays a key role in embryonic development and epigenetic reprogramming, and is specifically expressed in embryonic stem cells and germ-line cells. In mice, DPPA3, as a maternal factor, plays a role in the pre-implantation development stage, participating in transcription inhibition, cell division and cell pluripotency maintenance. The predicted molecular weight is 17 kDa and the observed molecular weight is 23 kDa.