DPP4/CD26 Recombinant antibody

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  WB analysis using 85159-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85159-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 85159-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 85159-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 85159-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 85159-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85159-3-RR (DPPIV/CD26 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DPP4/CD26 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85159-3-RR

Clone No.242823E10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

242823E10, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form

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Tested Applications

• Positive WB detected in: mouse liver tissue, Recombinant protein, mouse lung tissue, mouse kidney tissue
• Positive IHC detected in: mouse liver tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85159-3-RR targets DPP4/CD26 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DPP4/CD26 fusion protein Eg1384
• Full Name: dipeptidylpeptidase 4
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_010074.3
• Gene Symbol: Dpp4
• Gene ID (NCBI): 13482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28843
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).