DPYD Polyclonal antibody

• Home
• Products
• Primary Antibodies
• DPYD Polyclonal antibody (27662-1-AP)

DPYD Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27662-1-AP Copied!

Publications (11)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

DHP, DHPDHASE, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPD, DPYD

• 

  WB analysis of rat liver using 27662-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat liver using 27662-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 27662-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 27662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 27662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for DPYD in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: rat liver tissue, L02 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27662-1-AP targets DPYD in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26599

  Product name: Recombinant human DPYD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4-99 aa of BC008379

  Sequence: VLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKS

• Full Name: dihydropyrimidine dehydrogenase
• Calculated Molecular Weight: 1025 aa, 111 kDa
• Observed Molecular Weight: 111 kDa
• GenBank Accession Number: BC008379
• Gene Symbol: DPYD
• Gene ID (NCBI): 1806
• RRID: AB_2880941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12882
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The DPYD Polyclonal antibody (Cat# 27662-1-AP) has 11 citations published in journals including Nature Communications, Theranostics, Journal of Clinical Investigation, Cell Death & Differentiation, Cell Death & Disease, Journal of Ethnopharmacology, Journal of Cellular and Molecular Medicine, Discovery Oncology, Clinical and Translational Oncology, Journal of Traditional Chinese Medicine, and Pharmgenomics & Personalized Medicine. Research fields span glioma, hepatocellular carcinoma, breast cancer, colorectal cancer, pancreatic cancer, Fe-S cluster biology, and pyrimidine metabolism.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Nat Commun; Proteomic profiling of gliomas unveils immune and metabolism-driven subtypes with implications for anti-nucleotide metabolism therapy; Authors - Jinsen Zhang
human,mouse	IF	Theranostics; Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma; Authors - Yao Li
human	WB	J Clin Invest; CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes; Authors - Nunziata Maio
		Cell Death Differ; Partial bypass of frataxin deficiency by ISCU M141I restores cytosolic and nuclear Fe-S cluster assembly.; Authors - Valentine Mosbach
human	WB	Cell Death Dis; Everolimus destabilizes thymidylate synthase via suppressing its O-GlcNAcylation and sensitizes HER2-negative breast cancer to fluorouracil.; Authors - Xiao-Ting Jiang
mouse	WB	J Ethnopharmacol; Dabupi decoction mitigates fluorouracil-induced intestinal mucositis via farnesoid X receptor activation and dihydropyrimidine dehydrogenase upregulation.; Authors - ShunZhi Wang