CRMP4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CRMP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13661-1-AP

Synonyms

DPYSL3, DRP-3, DPYSL 3, Dihydropyrimidinase-related protein 3, CRMP-4

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Validation Data Gallery

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  WB analysis using 13661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13661-1-AP (CRMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13661-1-AP (CRMP4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13661-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13661-1-AP (CRMP4 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13661-1-AP targets CRMP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRMP4 fusion protein Ag4557
• Full Name: dihydropyrimidinase-like 3
• Calculated Molecular Weight: 570 aa, 62 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC039006
• Gene Symbol: CRMP4
• Gene ID (NCBI): 1809
• RRID: AB_10638641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPYSL3(Dihydropyrimidinase-related protein 3) is also named as CRMP4, DRP3, ULIP, ULIP1 and belongs to the hydantoinase/dihydropyrimidinase subfamily.DPYSL3 is a developmentally regulated protein, strongly expressed in early embryonic post-mitotic neural cells, reaching a peak of expression around the first post-natal week. In the adult brain, DPYSL3 is present mainly in regions that retain neurogenesis.DPYSL3 protein is also found in synaptic sites at the adult neuromuscular junction where it may be involved in the maintenance of neuronal stability(PMID: 16135096).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Mol Neurobiol; Collapsin response mediator protein 5 (CRMP5) modulates susceptibility to chronic social defeat stress in mice.; Authors - Yu-Fen Lin
mouse	WB	Neuropharmacology; Collapsin response mediator protein 5 (CRMP5) causes social deficits and accelerates memory loss in an animal model of Alzheimer's disease.; Authors - Yung-Shuen Lin
human	IF,IHC	Cancer Biol Ther; CRMP4a suppresses cell motility by sequestering RhoA activity in prostate cancer cells.; Authors - Changlin Li ; KD Validated
mouse	WB	Int J Mol Sci; Elevated Hippocampal CRMP5 Mediates Chronic Stress-Induced Cognitive Deficits by Disrupting Synaptic Plasticity, Hindering AMPAR Trafficking, and Triggering Cytokine Release; Authors - Yu-Fen Lin
human,rat	WB,IHC	Cell Rep Med; Bone transport induces the release of factors with multi-tissue regenerative potential for diabetic wound healing in rats and patients; Authors - Jing Xie