DR1 Polyclonal antibody

DR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10406-1-AP

Synonyms

DR1, NC2, NC2 BETA, Negative cofactor 2 beta, Protein Dr1

---

Validation Data Gallery

• 

  WB analysis of mouse testis using 10406-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10406-1-AP (DR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10406-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10406-1-AP (DR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10406-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10406-1-AP (DR1 antibody) at dilution of 1:10 (under 25x lens).

---

Tested Applications

• Positive WB detected in: mouse testis tissue, PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

10406-1-AP targets DR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DR1 fusion protein Ag0664
• Full Name: down-regulator of transcription 1, TBP-binding (negative cofactor 2)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC002809
• Gene Symbol: DR1
• Gene ID (NCBI): 1810
• RRID: AB_2094676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Down-regulator of transcription 1, TATA box-binding protein (TBP)-binding (negative cofactor 2) is a TBP associated phosphoprotein that represses both basal and activated levels of TBP transcription. DR1 is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. DR1 contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription.

Publications

Species	Application	Title
human	WB	Mol Cell Biol; The double-histone-acetyltransferase complex ATAC is essential for mammalian development.; Authors - Guelman Sebastián S
human	WB	Exp Cell Res; Dopamine D1 receptor activation ameliorates ox-LDL-induced endothelial cell senescence via CREB/Nrf2 pathway; Authors - Yiming Zhao