DR1 Polyclonal antibody

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• DR1 Polyclonal antibody (10406-1-AP)

• KD/KO Validated

DR1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10406-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Down-regulator of transcription 1, NC2, NC2 BETA, NC2-beta, Negative cofactor 2 beta

• 

  WB analysis of mouse testis using 10406-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10406-1-AP (DR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10406-1-AP

  WB result of DR1 antibody (10406-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DR1 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 10406-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10406-1-AP (DR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 10406-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10406-1-AP (DR1 antibody) at dilution of 1:10 (under 25x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, PC-3 cells, HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10406-1-AP targets DR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0664

  Product name: Recombinant human DR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-176 aa of BC002809

  Sequence: MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNKSEKKTISPEHVIQALESLGFGSYISEVKEVLQECKTVALKRRKASSRLENLGIPEEELLRQQQELFAKARQQQAELAQQEWLQMQQAAQQAQLAAASASASNQAGSSQDEEDDDDI

• Full Name: down-regulator of transcription 1, TBP-binding (negative cofactor 2)
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC002809
• Gene Symbol: DR1
• Gene ID (NCBI): 1810
• RRID: AB_2094676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01658
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Down-regulator of transcription 1, TATA box-binding protein (TBP)-binding (negative cofactor 2) is a TBP associated phosphoprotein that represses both basal and activated levels of TBP transcription. DR1 is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. DR1 contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription.

Publications

What published studies show

The DR1 Polyclonal antibody (Cat# 10406-1-AP) has a total of 2 citations. These appear in Experimental Cell Research (2023) and Molecular and Cellular Biology (2009). The cited studies span cardiovascular cell biology (dopamine D1 receptor-mediated endothelial senescence via CREB/Nrf2 pathway) and epigenetics/developmental biology (ATAC double-histone-acetyltransferase complex in mammalian development). Both citations used the antibody for Western Blot in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Exp Cell Res; Dopamine D1 receptor activation ameliorates ox-LDL-induced endothelial cell senescence via CREB/Nrf2 pathway; Authors - Yiming Zhao
human	WB	Mol Cell Biol; The double-histone-acetyltransferase complex ATAC is essential for mammalian development.; Authors - Guelman Sebastián S