DRD2 Polyclonal antibody

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  WB analysis using 55084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 55084-1-AP

  IP result of anti-DRD2 (IP:55084-1-AP, 4ug; Detection:55084-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 55084-1-AP (DRD2 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

DRD2 Polyclonal Antibody for WB, IF-P, IF-Fro, IP, ELISA

Cat No. 55084-1-AP

Publications(41)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Synonyms

D(2) dopamine receptor, D2 dopamine receptor, D2DR, D2R, Dopamine D2 receptor

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IF-P detected in: mouse brain tissue
• Positive IF-Fro detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 12 publications below

Product Information

55084-1-AP targets DRD2 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: dopamine receptor D2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_016574
• Gene Symbol: DRD2
• Gene ID (NCBI): 1813
• RRID: AB_10859941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14416
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
rat		Nat Commun; Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis; Authors - Nathan Bénac
mouse	WB	Mol Psychiatry; ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors.; Authors - Ying Huang
rat	WB	J Affect Disord; Pramipexole improves depression-like behavior in diabetes mellitus with depression rats by inhibiting NLRP3 inflammasome-mediated neuroinflammation and preventing impaired neuroplasticity; Authors - Ping Li
rat	WB	Int J Mol Sci; Biochemical Neuroadaptations in the Rat Striatal Dopaminergic System after Prolonged Exposure to Methamphetamine Self-Administration; Authors - Subramaniam Jayanthi
mouse	WB	J Neuroimmune Pharmacol; 5-HT4 Receptor is Protective for MPTP-induced Parkinson's Disease Mice Via Altering Gastrointestinal Motility or Gut Microbiota; Authors - Chun Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

ARUNASALAM (Verified Customer) (05-29-2025)

This antibody worked for western blotting.

• Applications: Western Blot
• Cell Tissue Type: Human Cancer Cell Lines

FH

Ping (Verified Customer) (06-04-2019)

This antibody is good for both immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: rat brain tissue