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  • KD/KO Validated

DRD2 Polyclonal antibody

DRD2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55084-1-AP

Synonyms

D(2) dopamine receptor, D2DR, D2R, Dopamine D2 receptor, dopamine receptor D2, DRD2



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IP detected inSH-SY5Y cells
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55084-1-AP targets DRD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dopamine receptor D2
Calculated Molecular Weight 51 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberNM_016574
Gene Symbol DRD2
Gene ID (NCBI) 1813
RRIDAB_10859941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

Protocols

Product Specific Protocols
WB protocol for DRD2 antibody 55084-1-APDownload protocol
IF protocol for DRD2 antibody 55084-1-APDownload protocol
IP protocol for DRD2 antibody 55084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

PMID: 25594175

Authors - Yiqing Yan
  • KD Validated
mouse

Mol Psychiatry

ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors.

Authors - Ying Huang
rat

Int J Mol Sci

Biochemical Neuroadaptations in the Rat Striatal Dopaminergic System after Prolonged Exposure to Methamphetamine Self-Administration

Authors - Subramaniam Jayanthi
mouse

J Neuroimmune Pharmacol

5-HT4 Receptor is Protective for MPTP-induced Parkinson's Disease Mice Via Altering Gastrointestinal Motility or Gut Microbiota

Authors - Chun Cui
humanIHC

Cancers (Basel)

Targeting Dopamine Receptor D2 by Imipridone Suppresses Uterine Serous Cancer Malignant Phenotype.

Authors - Wen Hu
mouse

Cancers (Basel)

Targeting Cancer Stem Cells with Differentiation Agents as an Alternative to Genotoxic Chemotherapy for the Treatment of Malignant Testicular Germ Cell Tumors.

Authors - Amanda R Loehr

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ping (Verified Customer) (06-04-2019)

This antibody is good for both immunoprecipitation and western blot.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: rat brain tissue