Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55084-1-AP targets DRD2 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | dopamine receptor D2 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | NM_016574 |
| Gene Symbol | DRD2 |
| Gene ID (NCBI) | 1813 |
| RRID | AB_10859941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14416 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DRD2 antibody 55084-1-AP | Download protocol |
| IP protocol for DRD2 antibody 55084-1-AP | Download protocol |
| WB protocol for DRD2 antibody 55084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DRD2 antibody (Cat# 55084-1-AP) has accumulated 65 citations published in journals including Cell, Nature Communications, Molecular Psychiatry, Advanced Science, Journal of Neuroinflammation, and International Journal of Molecular Sciences. Associated research fields encompass dopamine signaling, neurodegenerative diseases (Parkinson's, multiple system atrophy), neuropsychiatric disorders (depression, schizophrenia, ADHD), migraine and pain, addiction, endocrinology (prolactin regulation), oncology, and immunology. Primary applications include Western blot, immunofluorescence, and IHC.
| Species | Application | Title |
|---|---|---|
Adv Mater Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects | ||
Mol Neurodegener Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency | ||
Nat Commun Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis | ||
Adv Sci (Weinh) Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons | ||
J Immunother Cancer Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanoma. |
Reviews
What customers say
Users report this antibody performs well across multiple applications. One reviewer (5/5) used it successfully for immunofluorescence at 1:200 dilution for tagging validation and plans to order more. Another (3/5) confirmed it works for western blotting on human cancer cell lines. A third (5/5) found it effective for both immunoprecipitation and western blot at 1:500 on rat brain tissue. Overall, the antibody is considered reliable for WB and IP, with positive feedback on IF performance as well.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alexandre (Verified Customer) (01-31-2026) | Asked for a validation of tagging, works very well! will probably order a bit more for extra analysis
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ARUNASALAM (Verified Customer) (05-29-2025) | This antibody worked for western blotting.
|
Ping (Verified Customer) (06-04-2019) | This antibody is good for both immunoprecipitation and western blot.
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