Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, mouse brain tissue|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||mouse brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55084-1-AP targets DRD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||dopamine receptor D2|
|Calculated molecular weight||51 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||NM_016574|
|Gene ID (NCBI)||1813|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
DRD2, also named as D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.
|Product Specific Protocols|
|WB protocol for DRD2 antibody 55084-1-AP||Download protocol|
|IHC protocol for DRD2 antibody 55084-1-AP||Download protocol|
|IF protocol for DRD2 antibody 55084-1-AP||Download protocol|
|IP protocol for DRD2 antibody 55084-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Int Med Res
Mechanistic analysis of erectile dysfunction in a depression rat model.
BMC Endocr Disord
The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.
Clin Exp Pharmacol Physiol
Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF-β1/Smads and NFκB pathways.
Dopamine D2 receptors in the nucleus accumbens modulate erectile function in a rat model of nonorganic erectile dysfunction.
The ancient Chinese formula Longdan Xiegan Tang improves antipsychotic-induced hyperprolactinemia by repairing the hypothalamic and pituitary TGF-β1 signaling in rats.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ping (Verified Customer) (06-04-2019)
This antibody is good for both immunoprecipitation and western blot.