Tested Applications
| Positive WB detected in | HEK-293T cells, HEK-293 cells, Hela cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27958-1-AP targets DROSHA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27533 Product name: Recombinant human DROSHA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 353-550 aa of NM_001100412 Sequence: MHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHS Predict reactive species |
| Full Name | ribonuclease type III, nuclear |
| Calculated Molecular Weight | 159 aa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | NM_001100412 |
| Gene Symbol | DROSHA |
| Gene ID (NCBI) | 29102 |
| RRID | AB_2918137 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRR4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DROSHA antibody 27958-1-AP | Download protocol |
| IHC protocol for DROSHA antibody 27958-1-AP | Download protocol |
| WB protocol for DROSHA antibody 27958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DROSHA Polyclonal antibody (Cat# 27958-1-AP) has 3 citations, all using WB. They appear in Journal of Translational Medicine (cardiac fibrosis/m⁶A methylation, rat), Cell & Molecular Life Sciences (N4-acetylcytidine/miRNA processing in cancer, human), and PLoS Pathogens (avian leukosis virus/innate immunity, chicken). Research fields span RNA modification, miRNA biogenesis, cancer, and viral immunology.
| Species | Application | Title |
|---|---|---|
J Transl Med Nicotine promotes cardiac fibrosis by regulating miR-125b-5p maturation via HNRNPA2B1-mediated METTL14-dependent m⁶A methylation: therapeutic reversal by cinacalcet HCl. | ||
Cell Mol Life Sci N4-acetylcytidine modifies primary microRNAs for processing in cancer cells | ||
PLoS Pathog Avian leukosis virus subgroup J evades innate immunity by activating miR-155 to dually target TRAF3 and STAT1. |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody for Western Blot at a 1:1000 dilution with a 1-hour room-temperature incubation to detect DROSHA in HeLa cell extracts, reporting that it worked very well. Overall, the feedback highlights strong performance and reliability for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-15-2024) | Works very well to detect DROSHA by WB from HeLa extracts (1/1000 dilution, 1h incubation at room temperature).
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