DROSHA Polyclonal antibody

DROSHA Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 27958-1-AP

Synonyms

DROSHA, ETOHI2, HSA242976, p241, RANSE3L, Ribonuclease 3, Ribonuclease III, ribonuclease type III, nuclear, RN3, RNase III, RNASE3L, RNASEN

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Validation Data Gallery

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  WB analysis using 27958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27958-1-AP (DROSHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 27958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 27958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, Hela cells
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27958-1-AP targets DROSHA in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DROSHA fusion protein Ag27533
• Full Name: ribonuclease type III, nuclear
• Calculated Molecular Weight: 159 aa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_001100412
• Gene Symbol: RNASEN
• Gene ID (NCBI): 29102
• RRID: AB_2918137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.