DSC3 Polyclonal antibody

• 

  IHC staining of mouse skin using 31154-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31154-1-AP (DSC3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 31154-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 31154-1-AP (DSC3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DSC3 Polyclonal Antibody for IHC, ELISA

Cat No. 31154-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

CDHF3, desmocollin 3, Desmocollin 4, DSC, DSC1

---

Tested Applications

• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31154-1-AP targets DSC3 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DSC3 fusion protein Ag34648
• Full Name: desmocollin 3
• Calculated Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001941
• Gene Symbol: DSC3
• Gene ID (NCBI): 1825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q14574
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmocollin-3 (DSC3), also known as CDHF3 and DSC4, is a member of the cadherin superfamily of calcium-dependent cell adhesion molecules and a major component of desmosomes. It has 2 isoforms with molecular masses of 100 and 93 kDa. It is particularly expressed in the vagina, testis, skin, oral mucosa and esophagus. Its subcellular localization is at the cell membrane. Post-translational modifications, including glycosylation, have been described.