DSCR3 Polyclonal antibody

• 

  IP experiment of U-937 using 27243-1-AP

  IP result of anti-DSCR3 (IP:27243-1-AP, 4ug; Detection:27243-1-AP 1:500) with U-937 cells lysate 2160 ug.

• 

  IP experiment of SH-SY5Y using 27243-1-AP

  IP result of anti-DSCR3 (IP:27243-1-AP, 4ug; Detection:27243-1-AP 1:500) with SH-SY5Y cells lysate 1480 ug.

DSCR3 Polyclonal Antibody for IP, ELISA

Cat No. 27243-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Synonyms

DCRA, DSCRA

---

Tested Applications

• Positive IP detected in: U-937 cells, SH-SY5Y cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27243-1-AP targets DSCR3 in IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23350

  Product name: Recombinant human DSCR3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-297 aa of BC110655

  Sequence: MGTALDIKIKRANKVYHAGEVLSGVVVISSKDSVQHQGVSLTMEGTVNLQLSAKSVGVFEAFYNSVKPIQIINSTIEMVKPGKFPSGKTEIPFEFPLHLKGNKVLYETYHGVFVNIQYTLRCDMKRSLLAKDLTKTCEFIVHSAPQKGKFTPSPVDFTITPETLQNVKERALLPKFLLRGHLNSTNCVITQPLTGELVVESSEAAIRSVELQLVRVETCGCAEGYARDATEIQNIQIADGDVCRGLSVPIYMVFPRLFTCPTLETTNFKVEFEVNIVVLLHPDHLITENFPLKLCRI

• Full Name: Down syndrome critical region gene 3
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC110655
• Gene Symbol: DSCR3
• Gene ID (NCBI): 10311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14972
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DSCR3 (Down syndrome critical region protein 3) encodes VPS26C (Vacuolar protein sorting-associated protein 26C), a member of the retriever integral membrane protein recycling pathway. DSCR3 is a component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex (PMID: 31845315). DSCR3 specifically facilitates the transport of LRP1, but not of LDLR, from the endosomes to the plasma membrane, and hereby controls postprandial TG levels in plasma (PMID: 36353989, 35187626).