DSG1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84053-6-RR (same clone as 84053-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84053-6-RR (DSG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 84053-6-RR (same clone as 84053-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84053-6-RR (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 84053-6-RR (same clone as 84053-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84053-6-RR (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84053-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84053-6-RR against Human DSG1 were performed. The affinity constant is 71.2 pM.

DSG1 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84053-6-PBS

Clone No.241093G11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Desmosomal glycoprotein 1, Desmoglein-1, desmoglein 1, CDHF4, Cadherin family member 4

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Product Information

84053-6-PBS targets DSG1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20184

  Product name: Recombinant human DSG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 756-1049 aa of BC153001

  Sequence: KKLADISLGKESYPDLDPSWPPQSTEPVCLPQETEPVVSGHPPISPHFGTTTVISESTYPSGPGVLHPKPILDPLGYGNVTVTESYTTSDTLKPSVHVHDNRPASNVVVTERVVGPISGADLHGMLEMPDLRDGSNVIVTERVIAPSSSLPTSLTIHHPRESSNVVVTERVIQPTSGMIGSLSMHPELANAHNVIVTERVVSGAGVTGISGTTGISGGIGSSGLVGTSMGAGSGALSGAGISGGGIGLSSLGGTASIGHMRSSSDHHFNQTIGSASPSTARSRITKYSTVQYSK

• Full Name: desmoglein 1
• Calculated Molecular Weight: 1049 aa, 114 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC153001
• Gene Symbol: DSG1
• Gene ID (NCBI): 1828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q02413
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).