DSPP Polyclonal antibody

DSPP Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55396-1-AP

Synonyms

Dentin phosphophoryn, dentin sialophosphoprotein, DFNA39, DGI1, DMP3, DPP, DPP Dentin sialoprotein, DSP, DSPP, DTDP2

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Validation Data Gallery

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  WB analysis using 55396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55396-1-AP (DSPP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Caco-2 cells, LNCaP cells, MCF-7 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

55396-1-AP targets DSPP in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: dentin sialophosphoprotein
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_014208
• Gene Symbol: DSPP
• Gene ID (NCBI): 1834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. This antibody recognizes the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).

Publications

Species	Application	Title
human	WB	Int J Nanomedicine; Multifunctional Lithium-Doped Mesoporous Nanoparticles for Effective Dentin Regeneration in vivo; Authors - Zitian Liang
human	WB	Int Endod J; Effects of phase-transited lysozyme on adhesion, migration and odontogenic differentiation of human dental pulp cells: An in vitro study; Authors - Jiange Zhao