Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells, MCF-7 cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55396-1-AP targets DSPP in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | dentin sialophosphoprotein |
| Calculated Molecular Weight | 131 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | NM_014208 |
| Gene Symbol | DSPP |
| Gene ID (NCBI) | 1834 |
| RRID | AB_3086448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZW4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. The molecular mass of full-length is around 130kDa(uniprot), the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DSPP antibody 55396-1-AP | Download protocol |
| WB protocol for DSPP antibody 55396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
DSPP Polyclonal antibody (Cat# 55396-1-AP) has 13 citations published in journals including Nature Communications, Advanced Healthcare Materials, Regenerative Biomaterials, International Journal of Nanomedicine, International Journal of Biological Macromolecules, ACS Applied Materials & Interfaces, Stem Cell Research & Therapy, International Endodontic Journal, Colloids and Surfaces B, European Journal of Pharmacology, Cell Biochemistry and Biophysics, and Archives of Oral Biology. Citations span dentinogenesis, dental pulp regeneration, odontoblast differentiation, bone regeneration, and pulpitis therapy.
| Species | Application | Title |
|---|---|---|
Nat Commun Spatiotemporal interplay between epithelial and mesenchymal cells drives human dentinogenesis. | ||
Adv Healthc Mater Multifunctional Lithium-Calcium-Silicon Bioceramic Resin Composite for Enhanced Dental Remineralization and Reparative Dentin Formation | ||
Int J Biol Macromol Photopolymerizable fish collagen peptide-GelMa composite hydrogels for dental pulp regeneration. | ||
Regen Biomater Magnesium-doped bioactive glass enhances bone regeneration by reversing replicative senescence of human dental pulp stem cells in bone defect therapy. | ||
Int J Nanomedicine Multifunctional Lithium-Doped Mesoporous Nanoparticles for Effective Dentin Regeneration in vivo | ||
Stem Cell Res Ther Inhibition of miR-615-3p enhances dentinogenesis in scap(s) via PVT1-mediated mitochondrial regulation. |
