DSPP Polyclonal antibody

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  WB analysis using 55396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55396-1-AP (DSPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 55396-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DSPP antibody (55396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DSPP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 55396-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

Dentin phosphophoryn, dentin sialophosphoprotein, DFNA39, DGI1, DMP3, DPP, DPP Dentin sialoprotein, DSP, DSPP, DTDP2

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, MCF-7 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

55396-1-AP targets DSPP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: dentin sialophosphoprotein
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_014208
• Gene Symbol: DSPP
• Gene ID (NCBI): 1834
• RRID: AB_3086448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZW4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DSPP (dentin sialophosphoprotein) is a precursor protein belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. DSPP is secreted by odontoblasts and then is leaved into 3 fragments: N-terminal dentin sialoprotein (DSP), intermediate dentin glycoprotein (DGP) and C-terminal dentin phosphoprotein (DPP) (PMID:22201843). DSP is heavily glycosylated but DPP is not. DSP and DPP are principle proteins of the dentin extracellular matrix of the tooth, with DSP having a role in dentinogenesis and DPP binding calcium, facilitating initial mineralization of dentin matrix collagen and regulating the size and shape of the crystals. The molecular mass of full-length is around 130kDa(uniprot), the cleavage product of DSPP with a molecular mass of around 90 to 100 kDa (PMID: 21103065).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Nanomedicine; Multifunctional Lithium-Doped Mesoporous Nanoparticles for Effective Dentin Regeneration in vivo; Authors - Zitian Liang
human	WB	Arch Oral Biol; NOTUM plays a bidirectionally modulatory role in the odontoblastic differentiation of human stem cells from the apical papilla through the WNT/β-catenin signaling pathway; Authors - Qingxuan Zhao
mouse	WB	Eur J Pharmacol; Vdr mediates Wnt signaling pathway to regulate odontoblasts differentiation during dentin apposition; Authors - Yinlin Wu
human	WB	Int Endod J; Effects of phase-transited lysozyme on adhesion, migration and odontogenic differentiation of human dental pulp cells: An in vitro study; Authors - Jiange Zhao