DSTYK Polyclonal antibody

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  WB analysis using 20102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20102-1-AP (DSTYK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20102-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DSTYK antibody (20102-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

DSTYK Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20102-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RIPK5, Receptor-interacting serine/threonine-protein kinase 5, KIAA0472, HDCMD38P, EC:2.7.12.1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20102-1-AP targets DSTYK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13678

  Product name: Recombinant human DSTYK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-355 aa of BC060870

  Sequence: MEGDGVPWGSEPVSGPGPGGGGMIRELCRGFGRYRRYLGRLRQNLRETQKFFRDIKCSHNHTCLSSLTGGGGAERGPAGDVAETGLQAGQLSCISFPPKEEKYLQQIVDCLPCILILGQDCNVKCQLLNLLLGVQVLPTTKLGSEESCKLRRLRFTYGTQTRVSLALPGQYELVHTLVAHQGNWETIPEEDLEVQENNEDAAHVLAELEVTMHHALLQEVDVVVAPCQGLRPTVDVLGDLVNDFLPVITYALHKDELSERDEQELQEIRKYFSFPVFFFKVPKLGSEIIDSSTRRMESERSPLYRQLIDLGYLSSSHWNCGAPGQDTKAQSMLVEQSEKLRHLSTFSHQVLQTRL

• Full Name: dual serine/threonine and tyrosine protein kinase
• Calculated Molecular Weight: 66 kDa, 105 kDa
• Observed Molecular Weight: 66 kDa, 100~105 kDa
• GenBank Accession Number: BC060870
• Gene Symbol: DSTYK
• Gene ID (NCBI): 25778
• RRID: AB_10694146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6XUX3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DSTYK (Dual serine-threonine and tyrosine protein kinase gene), also known as CAKUT1, DustyPK, SPG23 has been classically considered as RIPK5, encodes a protein product consisting of 850-931 amino acids, which has a striking membrane-associated distribution in mesenchymal-derived cells of all major organs including brain, heart, kidney, lung, colon, and muscle (PMID: 34853310). DSTYK is strongly expressed in the cytoplasm of most malignant cells and that the high expression of DSTYK in human colon cancer specimens is associated with tumor progression and/or poor prognosis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
monkey	IF	Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
human,mouse	WB,IHC	Cell Death Dis; Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma; Authors - Chenxi Zhong ; KD Validated
human		EMBO Rep; DSTYK phosphorylates STING at late endosomes to promote STING signaling; Authors - Hao Dong