DTL Polyclonal antibody

DTL Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 12896-1-AP

Synonyms

CDT2, CDW1, DCAF2, Denticleless protein homolog, DTL, L2DTL, RAMP



Tested Applications

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells
Positive IP detected inmouse testis tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12896-1-AP targets DTL in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTL fusion protein Ag3589
Full Name denticleless homolog (Drosophila)
Calculated molecular weight 730 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC033540
Gene symbol DTL
Gene ID (NCBI) 51514
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

Protocols

Product Specific Protocols
WB protocol for DTL antibody 12896-1-APDownload protocol
IF protocol for DTL antibody 12896-1-APDownload protocol
IP protocol for DTL antibody 12896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols