DTL Polyclonal antibody

DTL Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12896-1-AP


CDT2, CDW1, DCAF2, Denticleless protein homolog, DTL, L2DTL, RAMP

Tested Applications

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells
Positive IP detected inmouse testis tissue
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12896-1-AP targets DTL in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTL fusion protein Ag3589
Full Name denticleless homolog (Drosophila)
Calculated Molecular Weight 730 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC033540
Gene Symbol DTL
Gene ID (NCBI) 51514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).


Product Specific Protocols
WB protocol for DTL antibody 12896-1-APDownload protocol
IF protocol for DTL antibody 12896-1-APDownload protocol
IP protocol for DTL antibody 12896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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