DTNA Polyclonal antibody

DTNA Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 10741-1-AP

Synonyms

Alpha dystrobrevin, D18S892E, DRP3, DTN, DTN A, DTNA, Dystrobrevin alpha, dystrobrevin, alpha, Dystrophin related protein 3, LVNC1

---

Validation Data Gallery

• 

  WB analysis using 10741-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10741-1-AP (DTNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10741-1-AP

  IP result of anti-DTNA (IP:10741-1-AP, 4ug; Detection:10741-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

---

Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10741-1-AP targets DTNA in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DTNA fusion protein Ag1075
• Full Name: dystrobrevin, alpha
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 25, 49-59 kDa, 78-85 kDa
• GenBank Accession Number: BC005300
• Gene Symbol: DTNA
• Gene ID (NCBI): 1837
• RRID: AB_2093895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DTNA (dystorbrevin alpha) is a cytoskeleton associated protein and an essential component of dystrophin protein complex. It is highly expressed in brain, heart and skeletal muscle. Alternative splicing generates several isoforms ranging from 22 to 84 kDa.