Dysbindin Polyclonal antibody

Dysbindin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11132-1-AP

Synonyms

DBND, DTNBP1, DTNBP1a, DTNBP1b, Dysbindin, Dysbindin 1, dystrobrevin binding protein 1, HPS7, HPS7 protein, My031, SDY

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Validation Data Gallery

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  WB analysis of mouse brain using 11132-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11132-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11132-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 11132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11132-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human testis tissue, mouse testis tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

11132-1-AP targets Dysbindin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Dysbindin fusion protein Ag1613
• Full Name: dystrobrevin binding protein 1
• Calculated Molecular Weight: 351 aa, 39 kDa
• Observed Molecular Weight: 48 kDa, 36 kDa
• GenBank Accession Number: BC011912
• Gene Symbol: Dysbindin
• Gene ID (NCBI): 84062
• RRID: AB_11182503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dysbindin, encoded by DTNBP1 (dystrobrevin binding protein 1), is a synaptic protein which is expressed in diverse neuronal populations throughout the brain. Genetic variation in DTNBP1 is associated with schizophrenia. Reduced expression of dysbindin has been reported in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia cases. Several isoforms of dysbindin exist: 48-50 kDa of dysbindin-1A, 35-38 kDa of dysbindin-1B, and 30-32 kDa of dysbindin-1C. This antibody recognizes all of isoforms of dysbindin. (19617633)

Publications

Species	Application	Title
		J Cell Biol; BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.; Authors - William J Monis
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook
mouse	WB	Neuropharmacology; Mice with dopaminergic neuron-specific deletion of DTNBP-1 gene show blunted nucleus accumbens dopamine release and associated behaviors.; Authors - Sanjeev K Bhardwaj
human	WB	Neuroscience; Protein kinase B/Akt1 phosphorylates dysbindin-1A at serine 10 to regulate neuronal development.; Authors - Erkang Fei
human	WB	Br J Pharmacol; Dopaminergic signaling and behavioral alterations by Comt-Dtnbp1 genetic interaction and their clinical relevance; Authors - Francesca Managò
human	WB	Sci Rep; Loss of dysbindin-1 in excitatory neurons in mice impacts NMDAR-dependent behaviors, neuronal morphology and synaptic transmission in the ventral hippocampus; Authors - Sanjeev K Bhardwaj