Tested Applications
| Positive IHC detected in | human testis tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18565-1-AP targets DTX2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4376 Product name: Recombinant human DTX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 322-622 aa of BC008856 Sequence: PMQMPKPSRVQQALAGMTSVLMSAIGLPVCLSRAPQPTSPPASRLASKSHGSVKRLRKMSVKEATPKPEPEPEQVIKNYTEELKVPPDEDCIICMEKLSTASGYSDVTDSKAIGSLAVGHLTKCSHAFHLLCLLAMYCNGNKDGSLQCPSCKTIYGEKTGTQPQGKMEVLRFQMSLPGHEDCGTILIVYSIPHGIQGPEHPNPGKPFTARGFPRQCYLPDNAQGRKVLELLKVAWKRRLIFTVGTSSTTGETDTVVWNEIHHKTEMDRNITGHGYPDPNYLQNVLAELAAQGVTEDCLEQQ Predict reactive species |
| Full Name | deltex homolog 2 (Drosophila) |
| Calculated Molecular Weight | 67 kDa |
| GenBank Accession Number | BC008856 |
| Gene Symbol | DTX2 |
| Gene ID (NCBI) | 113878 |
| RRID | AB_10643380 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UW9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DTX2 antibody 18565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DTX2 Polyclonal antibody (Cat# 18565-1-AP) has 1 citation. It appears in Cancer Science, a journal focused on oncology research. The cited study investigates how ubiquitination of NOTCH2 by DTX3 suppresses proliferation and migration in human esophageal carcinoma, placing this citation in the field of cancer biology, specifically esophageal cancer signaling pathways.
| Species | Application | Title |
|---|---|---|
Cancer Sci Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma. |
